LOCUS       sequence-133255-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Membrane tagged G-CaMP3 in cells expressing Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Vrontou S, Wong AM, Rau KK, Koerber HR, Anderson DJ
  TITLE     Genetic identification of C fibres that detect massage-like stroking
            of hairy skin in vivo.
  JOURNAL   Nature. 2013 Jan 31;493(7434):669-73. doi: 10.1038/nature11810.
  PUBMED    23364746
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2013-01-31- 31"; volume: "493"; issue: "7434"; 
            pages: "669-73"
COMMENT     Sequence Label: AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          95..570
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     protein_bind    complement(625..658)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(720..739)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctcccctc gaagcttaca
       61 tgtggtaccg agctcggatc ctgagaactt cagggtgagt ctatgggacc cttgatgttt
      121 tctttcccct tcttttctat ggttaagttc atgtcatagg aaggggagaa gtaacagggt
      181 acacatattg accaaatcag ggtaattttg catttgtaat tttaaaaaat gctttcttct
      241 tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct ttctttcagg
      301 gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa cagtgataat
      361 ttctgggtta aggcaatagc aatatttctg catataaata tttctgcata taaattgtaa
      421 ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca ttctgctttt
      481 attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct tttgctaatc
      541 atgttcatac ctcttatctt cctcccacag ctcctgggca acgtgctggt ctgtgtgctg
      601 gcccatcact ttggcaaaga attcataact tcgtatagca tacattatac gaagttatcg
      661 ccaccatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
      721 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
      781 tgtcagcgca ggggcgccc
//
