LOCUS       sequence-133264-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: Notch3 intracellular domain-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..90)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    98..122
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgac ttaacgcggt cattactacg tagaatcgag
       61 accgaggaga gggttaggga taggcttacc accggtaacc actttgtaca agaaagttgg
      121 caaggccaac acttgcctct tgggggtaac ttccggctgg ggccccagct gggtctgggc
      181 ctgagcaagg gagctgggaa cagacaaggg aagtggctgg gcaggcagtg ccccagtggt
      241 ggtggccatg gccccagtgg cagtggctgg gctaggcgtg gattcggacc agtctgagag
      301 ggagggaggt gaggggctgg cccagtgctc aggggattcg ggggatgggg tcaggtaagg
      361 gtgctcactg ggaacccgca ggaagcgggc ctttgggggg ctgctgtgtg ccccagccgc
      421 cgggtactcc tcgccatgtc ctgggactgc caggtaaggc gggggccgct cctgcgggga
      481 gacgggggtc cctgggttga gcagctgggg tcccggcgcc agtggcagca ggaacgaggg
      541 gcctggaggg gcaggtgggg gcagccgggc ccaatcgagg ggcacagcca cagggttcag
      601 caggcccagg ctgagtacac atcctccagg gggctggcgc cctagacccg cccggcctgg
      661 gccaccaagc tgtgccagag acactgcagt ggcagtggca gctgcatagg gcccctcaag
      721 ggggaagcca ccaggggaag cagggggccc accgaaaggc cgcggggagt ccagcgagtc
      781 cacgggcgac agcgtgaccg agctgtcagc cagggggccc gggcaggcca gcgtcagctt
      841 cttgccccgc ccccggggcc cctgcggccc cagccccgcc ttcccggggg gcctcctgct
      901 cttcttggac cccgactgtg ccgctttgag gccagggagg aaggcccctg gaggacagag
      961 cagaggcccc aggccgtggg gaccgggggg gctgcggggc ccactgggtt gatccagca
//
