LOCUS       sequence-133267-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: RalA (G23V)(mature peptide)-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(47..88)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    96..120
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(748..772)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttttgtaatc cagaggttga ttgtcgactt aacgcggtca ttactacgta gaatcgagac
       61 cgaggagagg gttagggata ggcttaccac cggtaaccac tttgtacaag aaagttggca
      121 agcatctttc tctgattctc ttggctaaac ttttcctctt cttttttcca ttcttttctt
      181 tgctgtcttc catctttctt gctcgaattt ctctcattaa atcaaaaaat accttgtcaa
      241 cattagctcg tgttttagca gacgtttcca cgtagttaac attccactgg tcagctctgt
      301 tttttgcctc ttctacagaa acctgccttt tatcttctaa atctgatttg ttaccaacca
      361 gtagaaatgg aacattctcg tcttctttta ctcttaaaat ctgctccctg aagtcagctg
      421 tagctgcaaa ggattccatt tctgtaatag agaagacaca gaggaagccc tccccgcttc
      481 ggaagtagtt gtctctaatt gcagcatagt cctcctgccc agctgtatct aagatatcaa
      541 tctgtacttc ctccccatcc agcactacct tcttccggta gctgtctgct ttggtaggct
      601 catagtcctc cacaaactca tcgtacatga actgtagagt cagagctgac ttgcccacac
      661 caacactgcc caccatgatg actttgtgta aggccaaaga attttgaccc ttgggcttat
      721 ttgcagccat ggtgccgccg ccgcaaaagc caactttttt gtacaaactt gttgatccat
      781 cgatgctagc ccctggggga gagaggtcgg tgattcggtc aacgagggag ccgactgccg
      841 acgtgcgctc cggaggcttg cagaatgcgg aagca
//
