LOCUS       sequence-133285-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE
  TITLE     One-step cloning and chromosomal integration of DNA.
  JOURNAL   ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. 
            Epub 2013 May 20.
  PUBMED    24050148
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400021j";
            journalName: "ACS Synth Biol"; date: "2013-09-20- 20"; volume: "2"; 
            issue: "9"; pages: "537-41"
COMMENT     Sequence Label: pOSIP-KO (KanR, 186)
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             155..163
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             175..197
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aattgcttta aggcgacgtg cgtcctcaag ctgctcttgt gttaatggtt tcttttttgt
       61 gctcatacgt taaatctatc accgcaaggg ataaatatct aacaccgtgc gtgttgacta
      121 ttttacctct ggcggtgata atggttgcat gtactaagga ggttgtatac gcgttttgtt
      181 taactttaag aaggagatat acatatgacc gtccgtaaaa atccggctgg cggttggatt
      241 tgtgaactct acccaaacgg tgcaaaaggc aaacgtatca gaaagaaatt cgctactaaa
      301 ggcgaggctc tggcgtttga gcagtacacc gttcaaaacc cgtggcagga agaaaaggaa
      361 gacaggcgca cgttaaaagg gctggttgat tcatggtata gcgctcatgg cattacactg
      421 aaagatggtt tgaaacgcca gttagccatg caccatgctt ttgagtgtat gggcgaacca
      481 ctcgcacgcg atttcgatgc gcagatgttt tcccgctacc gagaaaaacg gttaaaaggt
      541 gagtatgccc gttcaaacag agtgaaagag gtatcgcctc gcacgcttaa tcttgagctg
      601 gcctacttcc gggcggtgtt caatgagcta aaccgcctcg gagaatggaa gggtgaaaac
      661 ccactaaaaa atatgcgccc attccgcaca gaagaaatgg aaatgacttg gctaactcac
      721 gaccaaattt cgcaactgct cggagagtgt aaccgacatg accaccctga tttagaaacc
      781 gtggtaagaa tctgtctcgc aactggcgca cggtggtctg aggccgagag tctga
//
