LOCUS       sequence-133287-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV plasmid expressing the FlpO recombinase. with empty gRNA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  TITLE     Cas9/CRISPR pig transposon 
            plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-synt...
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..22
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcctaagaag aagaggaagg tgatgagcca gttcgacatc ctgtgcaaga ccccccccaa
       61 ggtgctggtg cggcagttcg tggagagatt cgagaggccc agcggcgaga agatcgccag
      121 ctgtgccgcc gagctgacct acctgtgctg gatgatcacc cacaacggca ccgccatcaa
      181 gagggccacc ttcatgagct acaacaccat catcagcaac agcctgagct tcgacatcgt
      241 gaacaagagc ctgcagttca agtacaagac ccagaaggcc accatcctgg aggccagcct
      301 gaagaagctg atccccgcct gggagttcac catcatccct tacaacggcc agaagcacca
      361 gagcgacatc accgacatcg tgtccagcct gcagctgcag ttcgagagca gcgaggaggc
      421 cgacaagggc aacagccaca gcaagaagat gctgaaggcc ctgctgtccg agggcgagag
      481 catctgggag atcaccgaga agatcctgaa cagcttcgag tacaccagca ggttcaccaa
      541 gaccaagacc ctgtaccagt tcctgttcct ggccacattc atcaactgcg gcaggttcag
      601 cgacatcaag aacgtggacc ccaagagctt caagctggtg cagaacaagt acctgggcgt
      661 gatcattcag tgcctggtga ccgagaccaa gacaagcgtg tccaggcaca tctactt
//
