LOCUS       sequence-133289-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a catalytically inactive form of the endonuclease ISceI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Roukos V, Voss TC, Schmidt CK, Lee S, Wangsa D, Misteli T
  TITLE     Spatial dynamics of chromosome translocations in living cells.
  JOURNAL   Science. 2013 Aug 9;341(6146):660-4. doi: 10.1126/science.1237150.
  PUBMED    23929981
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2013-08-9- 9"; volume: "341"; issue: 
            "6146"; pages: "660-4"
COMMENT     Sequence Label: HA-ISceID44A
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..73
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             95..115
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             134..160
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tttggcagat tcctcgaaat tcagatccgc cgccactatg ggatcaagat cgccaaaaaa
       61 gaagagaaag gtgccgaaga agcatgcagc accaccaaaa aaaaaacgaa aagtagaaga
      121 cccacgattt atgtacccat acgatgttcc tgactatgcg ggtatgaaaa acatcaaaaa
      181 aaaccaggta atgaacctgg gtccgaactc taaactgctg aaagaataca aatcccagct
      241 gatcgaactg aacatcgaac agttcgaagc aggtatcggt ctgatcctgg gtgctgctta
      301 catccgttct cgtgatgaag gtaaaaccta ctgtatgcag ttcgagtgga aaaacaaagc
      361 atacatggac cacgtatgtc tgctgtacga tcagtgggta ctgtccccgc cgcacaaaaa
      421 agaacgtgtt aaccacctgg gtaacctggt aatcacctgg ggcgcccaga ctttcaaaca
      481 ccaagctttc aacaaactgg ctaacctgtt catcgttaac aacaaaaaaa ccatcccgaa
      541 caacctggtt gaaaactacc tgaccccgat gtctctggca tactggttca tggatgatgg
      601 tggtaaatgg gattacaaca aaaactctac caacaaatcg atcgtactga acacccagtc
      661 tttcactttc gaagaagtag aatacctggt taagggtctg cgtaacaaat tccaactgaa
      721 ctgttacgta aaaatcaaca aaaacaaacc gatcatctac atcgattcta tgtcttacct
      781 gatcttctac aacctgatca aaccgtacct gatcccgcag atgatgtaca
//
