LOCUS       sequence-133298-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: MEK1 (S218D,S222D)-pcw107
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgac ttaacgcgtt cattactaac cggtaaccac
       61 tttgtacaag aaagttggca attagacgcc agcagcatgg gttggtgtgc tgggctggtt
      121 aaggccgatg gtggagcaga gccaacctgc aaaatccact tcctcagcat cagatctctt
      181 gataaaagca tgaaccatga gttgcttcaa atctgctctc tctgcggggt tttttattaa
      241 gcatttattc acaaaatctt gaaattccag actgaacact ccactgggca gttttggagg
      301 aggctcgttg actatgtaat ccaacaactc aaaaattgcc atgggaggtc ggctgtccat
      361 tccgtatgag ctaaggggcc tcccgggggt ccttggcctg ggtggggtct cagccgcatc
      421 tccttccacc tggcacccaa acatcagctc cagctccttg gcatctggag gagggatggg
      481 atacctccca accgccatct ctaccagaga cagtcccatg ctccagatgt ctgactgcac
      541 agagtaatga gtcccctgga gtctttctgg cgacatgtag gaccttgtgc ccacgaagtc
      601 gttggccatg tcgtcgatga gctgcccgct gaccccaaag tcacagagct tgatctcccc
      661 acgggagttg actaggatgt tggagggctt gacatctctg tgcatgatct tgtgcttctc
      721 cctcagatat gtcaggcctt ttattacagc aatgctaact tttcctaaaa tttgttcagg
      781 aattcttcca gctttcttca ggacttgatc cagagaacct ccatccatgt gctccatgca
      841 gatactgatc tcgccatcgc tgtagaacgc accatagaag cccacgatgt acggagagtt
      901 gcactcatgc agaacctgca gctcccttat gatctggttc cggattgcgg gtttgatctc
      961 cagatgaatt agctttctgg ccatgacc
//
