LOCUS       sequence-133302-         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Egg cell-specific promoter-controlled expression of 
            3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target
            sequence (U6-26 promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Wang ZP, Xing HL, Dong L, Zhang HY, Han CY, Wang XC, Chen QJ
  TITLE     Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently 
            generates homozygous mutants for multiple target genes in 
            Arabidopsis in a single generation.
  JOURNAL   Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.
  PUBMED    26193878
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-07-21"; volume: "16:144. doi"; pages: " 
            10.1186/s13059-015-0715-0"
COMMENT     Sequence Label: pHEE401
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(300..320)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(327..392)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 accttcgcca tctcgttgga gaaaatctcc tgcaggtagc agatgcgatt cttcctgcgc
       61 gtgtaccgcc tgcgggcggt gcgcttgagc cgcgtagcct cagccgtctc gccgctgtcg
      121 aacaggagag cgccaatgag attcttcttg atggaatgcc gatcggtgtt gcccaggacc
      181 ttgaacttct ttgagggcac cttgtactcg tcggtgatca cggcccagcc aacagagtta
      241 gtcccaatat cgaggccgat cgagtacttc ttgtcagcag ctggcacccc gtggatgcca
      301 accttcctct tcttcttcgg agccatcttg tcatcatcat ccttgtaatc aatgtcgtgg
      361 tccttgtaat ccccgtcgtg gtccttgtaa tccatctagc ttattctttc tttttggggt
      421 ttttgttttg ttttgttttg ttttgcgatg attgagttac gagagttctg tttttatatt
      481 gtggttttgg atggattcga agcttagtgg tgatttaaga gtaattaaaa agttggatta
      541 gtgggaggaa tgggaaagga ttgacggtaa atagtcactt aatcatgaaa cgttaaagca
      601 tttgactgta gaaacgtatt tagtatatat tggcatgttg aatcagtagc gctcctattg
      661 gagacgaagg tttaacgtcg tgttacagaa agtgaaccaa tgcttttgta ataaactcat
      721 tgaactttat gctatatact gtatatggtt t
//
