LOCUS       sequence-133304-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Egg cell-specific promoter-controlled expression of 
            3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target
            sequence (U6-26 promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Wang ZP, Xing HL, Dong L, Zhang HY, Han CY, Wang XC, Chen QJ
  TITLE     Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently 
            generates homozygous mutants for multiple target genes in 
            Arabidopsis in a single generation.
  JOURNAL   Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.
  PUBMED    26193878
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-07-21"; volume: "16:144. doi"; pages: " 
            10.1186/s13059-015-0715-0"
COMMENT     Sequence Label: pHEE401
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atcatttctt cttagctttt tttcttcttc ttcgttcata cagttttttt ttgtttatca
       61 gcttacattt tcttgaaccg tagctttcgt tttcttcttt ttaactttcc attcggagtt
      121 tttgtatctt gtttcatagt ttgtcccagg attagaatga ttaggcatcg aaccttcaag
      181 aatttgattg aataaaacat cttcattctt aagatatgaa gataatcttc aaaaggcccc
      241 tgggaatctg aaagaagaga agcaggccca tttatatggg aaagaacaat agtatttctt
      301 atataggccc atttaagttg aaaacaatct tcaaaagtcc cacatcgctt agataagaaa
      361 acgaagctga gtttatatac agctagagtc gaagtagtga ttgggagacc aacccagtgg
      421 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg
      481 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
      541 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
      601 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
      661 cctaaaacaa agttaaacat catgggggaa gcggtgatcg ccgaagtatc gactcaacta
      721 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
      781 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
      841 gtgaccgta
//
