LOCUS       sequence-133308-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mGFP and Synaptophysin-mRuby expression from Cre-expressing neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Beier KT, Steinberg EE, DeLoach KE, Xie S, Miyamichi K, Schwarz L, 
            Gao XJ, Kremer EJ, Malenka RC, Luo L
  TITLE     Circuit Architecture of VTA Dopamine Neurons Revealed by Systematic 
            Input-Output Mapping.
  JOURNAL   Cell. 2015 Jul 30;162(3):622-34. doi: 10.1016/j.cell.2015.07.015.
  PUBMED    26232228
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.07"; journalName: "Cell"; date: "2015-07-30- 
            30"; volume: "162"; issue: "3"; pages: "622-34"
COMMENT     Sequence Label: pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    81..114
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    177..210
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgcagtcgag aaggtaccgg atcctgtgct gtctcatcat tttggcaaag aattgatttg
       61 ataccgcggg cccggccggg ataacttcgt ataaagtatc ctatacgaag ttatatcaaa
      121 ataggaagac caatgcttca ccatcgaccc gaattgccaa gcatcaccat cgacacataa
      181 cttcgtataa tgtatgctat acgaagttat gtcgacttac cctccgccca ggccggcgaa
      241 cttggccacg gcgtgctccc gctgcaccac gaacatctcg ttgtcggact cttccagcct
      301 ttccagccgg tggtccacgg cgtggatgcc gggcatcttg atgttgccca cggttttctt
      361 ggatcggtag gtggtcacga agctgcagct caggtgccct ccgccgtcca ccttcagggc
      421 catgtgggtg tagcctctca gtccgccgtc agcggggtac atcatctcgg tgttgggctc
      481 ccagcccttg gttttcttct gcatcacggc gccgttgctg ggaaaattca cgcccctgac
      541 ctgggcatgg tacaccaggc agccatcttc caggctggtg tcctgcatca cggtgatcac
      601 gccgccgtcc tcgtatctgg tcactctctc ccaggtgaag ccctcgggga agctctgctt
      661 gaagaaatcg gggatgccct tggggtactt gatgaaggtc cggctgccgt acatgaagga
      721 tgtggccagg atgtcgaagg cgaagggcag aggtccgccc tcgatcactt tgatccgcat
      781 ggtctgggtg cc
//
