LOCUS       sequence-133309-         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mGFP and Synaptophysin-mRuby expression from Flp-expressing neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Beier KT, Steinberg EE, DeLoach KE, Xie S, Miyamichi K, Schwarz L, 
            Gao XJ, Kremer EJ, Malenka RC, Luo L
  TITLE     Circuit Architecture of VTA Dopamine Neurons Revealed by Systematic 
            Input-Output Mapping.
  JOURNAL   Cell. 2015 Jul 30;162(3):622-34. doi: 10.1016/j.cell.2015.07.015.
  PUBMED    26232228
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.07"; journalName: "Cell"; date: "2015-07-30- 
            30"; volume: "162"; issue: "3"; pages: "622-34"
COMMENT     Sequence Label: pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..119
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 aggtaccgga tcctgtgctg tctcatcatt ttggcaaaga attgatttga taccgcgggc
       61 ccgggggtac cgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttca
      121 tcaaaatagg aagaccaatg cttcaccatc gacccgaatt gccaagcatc accatcgaca
      181 gaagttccta ttccgaagtt cctattcttc aaaaggtata ggaacttcgt cgacttaccc
      241 tccgcccagg ccggcgaact tggccacggc gtgctcccgc tgcaccacga acatctcgtt
      301 gtcggactct tccagccttt ccagccggtg gtccacggcg tggatgccgg gcatcttgat
      361 gttgcccacg gttttcttgg atcggtaggt ggtcacgaag ctgcagctca ggtgccctcc
      421 gccgtccacc ttcagggcca tgtgggtgta gcctctcagt ccgccgtcag cggggtacat
      481 catctcggtg ttgggctccc agcccttggt tttcttctgc atcacggcgc cgttgctggg
      541 aaaattcacg cccctgacct gggcatggta caccaggcag ccatcttcca ggctggtgtc
      601 ctgcatcacg gtgatcacgc cgccgtcctc gtatctggtc actctctccc aggtgaagcc
      661 ctcggggaag ctctgcttga agaaatcggg gatgcccttg gggtacttga tgaaggtccg
      721 gctgccgtac atgaaggatg tggccaggat gtcga
//
