LOCUS       sequence-133311-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the amyloid-beta protein (42 aa), containing an exogenous 
            methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. 
            coli cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Walsh DM, Thulin E, Minogue AM, Gustavsson N, Pang E, Teplow DB, 
            Linse S
  TITLE     A facile method for expression and purification of the Alzheimer's 
            disease-associated amyloid beta-peptide.
  JOURNAL   FEBS J. 2009 Mar;276(5):1266-81. doi: 
            10.1111/j.1742-4658.2008.06862.x.
  PUBMED    19175671
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1742-4658.2008.06862"; journalName: "FEBS J"; date: 
            "2009-03"; volume: "276"; issue: "5"; pages: "1266-81"
COMMENT     Sequence Label: pET-Sac-Abeta(M1-42)
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(207..225)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      221..268
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(634..639,640..656,657..662))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(689..707)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agaaggagat atacatatgg acgctgaatt ccgtcacgac tctggttacg aagttcacca
       61 ccagaagctg gtgttcttcg ctgaagacgt gggttctaac aagggtgcta tcatcggtct
      121 gatggttggt ggcgttgtga tcgcttaata ggagctcgat ccggctgcta acaaagcccg
      181 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
      241 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca
      301 ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca
      361 ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt
      421 gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga
      481 cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca
      541 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac
      601 tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc
      661 aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
      721 gtcatgataa taatggtttc ttagacgtca ggtggcactt t
//
