LOCUS       sequence-133312-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the amyloid-beta protein (40 aa), containing an exogenous 
            methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. 
            coli cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Walsh DM, Thulin E, Minogue AM, Gustavsson N, Pang E, Teplow DB, 
            Linse S
  TITLE     A facile method for expression and purification of the Alzheimer's 
            disease-associated amyloid beta-peptide.
  JOURNAL   FEBS J. 2009 Mar;276(5):1266-81. doi: 
            10.1111/j.1742-4658.2008.06862.x.
  PUBMED    19175671
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1742-4658.2008.06862"; journalName: "FEBS J"; date: 
            "2009-03"; volume: "276"; issue: "5"; pages: "1266-81"
COMMENT     Sequence Label: pET-Sac-Abeta(M1-40)
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(201..219)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      215..262
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(628..633,634..650,651..656))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(683..701)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agaaggagat atacatatgg acgctgaatt ccgtcacgac tctggttacg aagttcacca
       61 ccagaagctg gtgttcttcg ctgaagacgt gggttctaac aagggtgcta tcatcggtct
      121 gatggttggt ggcgttgtgt aataggagct cgatccggct gctaacaaag cccgaaagga
      181 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
      241 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg
      301 gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg
      361 ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca
      421 acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat
      481 cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga
      541 cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt
      601 agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat
      661 gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
      721 ataataatgg tttcttagac gtcaggtggc actt
//
