LOCUS       sequence-133313-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of mCherry-LacR-BRCA1 siRNA resistant 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker 
            A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, 
            Durocher D
  TITLE     A mechanism for the suppression of homologous recombination in G1 
            cells.
  JOURNAL   Nature. 2015 Dec 9. doi: 10.1038/nature16142.
  PUBMED    26649820
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Dec 9. doi: 10.1038/nature16142."
COMMENT     Sequence Label: pDEST-mCherry-LacR-BRCA1
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             514..534
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    544..568
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa
       61 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc
      121 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg
      181 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg
      241 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc
      301 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag
      361 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc
      421 ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      481 gcacgacagg tttcccgaag cagcctgagg cctcctaaga agaagaggaa ggttggcgcg
      541 cccacaagtt tgtacaaaaa agcaggcttc accatggatt tatctgctct tcgcgttgaa
      601 gaagtacaaa atgtcattaa tgctatgcag aaaatcttag agtgtcccat ctgtctggag
      661 ttgatcaagg aacctgtctc cacaaagtgt gaccacatat tttgcaaatt ttgcatgctg
      721 aaacttctca accagaagaa
//
