LOCUS       sequence-133314-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP 
            expression cassette - Ampicillin plasmid 
            [A:J23102:B:BCD2:C:E1010m:D:B0015:E].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: pJ02B2Rm_AE
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      114..185
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      201..228
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    246..266
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(272..289)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(523..542)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaaccgacat caaactggac atcacctccc acaacgagga ctacaccatc gttgaacagt
       61 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata aaggtccagg catcaaataa
      121 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      181 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatagcttag
      241 agacctacta gtagcggccg ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg
      301 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      361 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      421 aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa
      481 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      541 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      601 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      661 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      721 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      781 cgccactggc agcagccact ggt
//
