LOCUS       sequence-133316-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mGFP and Synaptophysin-mRuby expression from Cre-expressing neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Beier KT, Steinberg EE, DeLoach KE, Xie S, Miyamichi K, Schwarz L, 
            Gao XJ, Kremer EJ, Malenka RC, Luo L
  TITLE     Circuit Architecture of VTA Dopamine Neurons Revealed by Systematic 
            Input-Output Mapping.
  JOURNAL   Cell. 2015 Jul 30;162(3):622-34. doi: 10.1016/j.cell.2015.07.015.
  PUBMED    26232228
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.07"; journalName: "Cell"; date: "2015-07-30- 
            30"; volume: "162"; issue: "3"; pages: "622-34"
COMMENT     Sequence Label: pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    63..96
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    167..200
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             join(273..275,276..278,279..791)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(318..339)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(579..598)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atccagaggt tgattatcga taagcttgat atcgaattca tcgatcggcc gcatataagc
       61 ttataacttc gtataatgta tgctatacga agttatgggt cgatggtgag atctggacta
      121 gagggtcgat ggtgatgctt ggatcaaaat aggaagaatc ccaatgataa cttcgtataa
      181 agtatcctat acgaagttat aggcgcgcca ccatgctgtg ctgtatgaga agaaccaaac
      241 aggttgaaaa gaatgatgag gaccaaaaga tcatggtgag caagggcgag gagctgttca
      301 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      361 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      421 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
      481 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      541 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      601 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      661 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      721 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
      781 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acc
//
