LOCUS       sequence-133322-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for the analysis of miR-21 activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Shen J, Xia W, Khotskaya YB, Huo L, Nakanishi K, Lim SO, Du Y, Wang 
            Y, Chang WC, Chen CH, Hsu JL, Wu Y, Lam YC, James BP, Liu X, Liu CG,
            Patel DJ, Hung MC
  TITLE     EGFR modulates microRNA maturation in response to hypoxia through 
            phosphorylation of AGO2.
  JOURNAL   Nature. 2013 May 16;497(7449):383-7. doi: 10.1038/nature12080. Epub 
            2013 May 1.
  PUBMED    23636329
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12080"; journalName: "Nature"; date: "2013-05-16- 
            16"; volume: "497"; issue: "7449"; pages: "383-7"
COMMENT     Sequence Label: pMIR-21 Reporter
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(209..226)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(209..225)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(218..240)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(434..453)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     553..575
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag
       61 gccaagaagg gcggaaagtc caaattgctc gagtgatgaa agctgcgcac tagttcaaca
      121 tcagtctgat aagctaaagc ttaataaagg atcttttatt ttcattggat ctgtgtgttg
      181 gttttttgta tgcggccgct agcttggcac tggccgtcgt tttacaacgt cgtgactggg
      241 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
      301 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      361 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
      421 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
      481 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
      541 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
      601 cgagacgaaa gattggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      661 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
      721 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
      781 catcccgccc ctaactccgc ccagttccgc c
//
