LOCUS       sequence-133323-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for the analysis of miR-21 activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Shen J, Xia W, Khotskaya YB, Huo L, Nakanishi K, Lim SO, Du Y, Wang 
            Y, Chang WC, Chen CH, Hsu JL, Wu Y, Lam YC, James BP, Liu X, Liu CG,
            Patel DJ, Hung MC
  TITLE     EGFR modulates microRNA maturation in response to hypoxia through 
            phosphorylation of AGO2.
  JOURNAL   Nature. 2013 May 16;497(7449):383-7. doi: 10.1038/nature12080. Epub 
            2013 May 1.
  PUBMED    23636329
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12080"; journalName: "Nature"; date: "2013-05-16- 
            16"; volume: "497"; issue: "7449"; pages: "383-7"
COMMENT     Sequence Label: pMIR-21 Reporter
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     156..178
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     170..187
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     171..187
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
       61 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
      121 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
      181 ggccagtgcc aagctagcgg ccgcatacaa aaaaccaaca cacagatcca atgaaaataa
      241 aagatccttt attaagcttt agcttatcag actgatgttg aactagtgcg cagctttcat
      301 cactcgagca atttggactt tccgcccttc ttggccttta tgaggatctc tctgattttt
      361 cttgcgtcga gttttccggt aagacctttc ggtacttcgt ccacaaacac aactcctccg
      421 cgcaactttt tcgcggttgt tacttgactg gcgacgtaat ccacgatctc tttttccgtc
      481 atcgtctttc cgtgctccaa aacaacaacg gcggcgggaa gttcaccggc gtcatcgtcg
      541 ggaagacctg ccacgcccgc gtcgaagatg ttggggtgtt gtaacaatat cgattccaat
      601 tcagcggggg ccacctgata tcctttgtat ttaattaaag acttcaagcg gtcaactatg
      661 aagaagtgtt cgtcttcgtc ccagtaagct atgtctccag aatgtagcca tccatccttg
      721 tcaatcaagg cgttggtcgc ttccggattg tttacataac cggacataat cataggtcct
      781 ctgacacata attcgcctct ctgattaacg cccagcgttt
//
