LOCUS       sequence-133325-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for the analysis of miR-31 activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Shen J, Xia W, Khotskaya YB, Huo L, Nakanishi K, Lim SO, Du Y, Wang 
            Y, Chang WC, Chen CH, Hsu JL, Wu Y, Lam YC, James BP, Liu X, Liu CG,
            Patel DJ, Hung MC
  TITLE     EGFR modulates microRNA maturation in response to hypoxia through 
            phosphorylation of AGO2.
  JOURNAL   Nature. 2013 May 16;497(7449):383-7. doi: 10.1038/nature12080. Epub 
            2013 May 1.
  PUBMED    23636329
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12080"; journalName: "Nature"; date: "2013-05-16- 
            16"; volume: "497"; issue: "7449"; pages: "383-7"
COMMENT     Sequence Label: pMIR-31-Reporter
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(208..225)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(208..224)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(217..239)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(433..452)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     552..574
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag
       61 gccaagaagg gcggaaagtc caaattgctc gagtgatgaa agctgcgcac tagtagctat
      121 gccagcatct tgcctaagct taataaagga tcttttattt tcattggatc tgtgtgttgg
      181 ttttttgtat gcggccgcta gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
      241 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
      301 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
      361 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
      421 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
      481 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
      541 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
      601 gagacgaaag attggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
      661 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      721 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgcc
//
