LOCUS       sequence-133334-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for the analysis of miR-31 activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Shen J, Xia W, Khotskaya YB, Huo L, Nakanishi K, Lim SO, Du Y, Wang 
            Y, Chang WC, Chen CH, Hsu JL, Wu Y, Lam YC, James BP, Liu X, Liu CG,
            Patel DJ, Hung MC
  TITLE     EGFR modulates microRNA maturation in response to hypoxia through 
            phosphorylation of AGO2.
  JOURNAL   Nature. 2013 May 16;497(7449):383-7. doi: 10.1038/nature12080. Epub 
            2013 May 1.
  PUBMED    23636329
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12080"; journalName: "Nature"; date: "2013-05-16- 
            16"; volume: "497"; issue: "7449"; pages: "383-7"
COMMENT     Sequence Label: pMIR-31-Reporter
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     156..178
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     170..187
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     171..187
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
       61 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
      121 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
      181 ggccagtgcc aagctagcgg ccgcatacaa aaaaccaaca cacagatcca atgaaaataa
      241 aagatccttt attaagctta ggcaagatgc tggcatagct actagtgcgc agctttcatc
      301 actcgagcaa tttggacttt ccgcccttct tggcctttat gaggatctct ctgatttttc
      361 ttgcgtcgag ttttccggta agacctttcg gtacttcgtc cacaaacaca actcctccgc
      421 gcaacttttt cgcggttgtt acttgactgg cgacgtaatc cacgatctct ttttccgtca
      481 tcgtctttcc gtgctccaaa acaacaacgg cggcgggaag ttcaccggcg tcatcgtcgg
      541 gaagacctgc cacgcccgcg tcgaagatgt tggggtgttg taacaatatc gattccaatt
      601 cagcgggggc cacctgatat cctttgtatt taattaaaga cttcaagcgg tcaactatga
      661 agaagtgttc gtcttcgtcc cagtaagcta tgtctccaga atgtagccat ccatccttgt
      721 caatcaaggc gttggtcgct tccggattgt ttacataacc ggacataatc ataggtcctc
      781 tgacacataa ttcgcctctc tgattaacgc ccagcgttt
//
