LOCUS       sequence-133337-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet inducible, Gateway adapted ROSA26 targeting Vector with DTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Melidoni AN, Dyson MR, Wormald S, McCafferty J
  TITLE     Selecting antagonistic antibodies that control differentiation 
            through inducible expression in embryonic stem cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Oct 29;110(44):17802-7. doi: 
            10.1073/pnas.1312062110. Epub 2013 Sep 30.
  PUBMED    24082130
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1312062110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-10-29- 29"; volume: "110"; issue: "44"; pages: "17802-7"
COMMENT     Sequence Label: pGATOR-DTA
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(109..233)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    380..461
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(417..436)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(626..649)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
       61 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
      121 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
      181 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgatatcg
      241 gtaccgataa gcttgtcgac gatatctcta gaggatcata atcagccata ccacatttgt
      301 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
      361 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
      421 tagcatcaca aatttcacaa ataaagcatt tttttcactg cctcgagctt ctctcctgac
      481 tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc
      541 tagagcggcc gctagctcga gtcgatctga tatcatcgat gaattctacc gggtagggga
      601 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
      661 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
      721 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
      781 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
      841 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggcctt
//
