LOCUS       sequence-133341-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet inducible, Gateway adapted ROSA26 targeting Vector with DTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Melidoni AN, Dyson MR, Wormald S, McCafferty J
  TITLE     Selecting antagonistic antibodies that control differentiation 
            through inducible expression in embryonic stem cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Oct 29;110(44):17802-7. doi: 
            10.1073/pnas.1312062110. Epub 2013 Sep 30.
  PUBMED    24082130
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1312062110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-10-29- 29"; volume: "110"; issue: "44"; pages: "17802-7"
COMMENT     Sequence Label: pGATOR-DTA
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(80..86,87..104,105..110))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(135..259)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(285..599)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(288..312)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    359..377
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    395..413
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    430..448
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    466..484
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    502..520
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    537..555
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    573..591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgacgga
       61 tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa gcctggggtg
      121 cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
      181 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
      241 cttttttgta caaacttgtg atatcggtac ccggggatcc tccaggcgat ctgacggttc
      301 actaaacgag ctctgcttat ataggcctcc caccgtacac gcctacctcg acatacgttc
      361 tctatcactg atagggagta aactcgacat acgttctcta tcactgatag ggataaactc
      421 gacatacgtt ctctatcact gatagggagt aaactcgaca tacgttctct atcactgata
      481 gggagtaaac tcgacatacg ttctctatca ctgataggga gtaaactcga catcgttctc
      541 tatcactgat agggagtaaa ctcgacatac gttctctatc actgataggg agtaaactcg
      601 agtgaagacg aagcttcctt agaaatctcg ccaagcttgg tcgagctgga tacttcccgt
      661 ccgccagggg gacatgccgg cgatgctgaa ggtcgcgcgc attcccgatg aagaggccgg
      721 ttaccgcctg ttgacctggt g
//
