LOCUS       sequence-133342-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for the analysis of miR-19-alpha 
            activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Shen J, Xia W, Khotskaya YB, Huo L, Nakanishi K, Lim SO, Du Y, Wang 
            Y, Chang WC, Chen CH, Hsu JL, Wu Y, Lam YC, James BP, Liu X, Liu CG,
            Patel DJ, Hung MC
  TITLE     EGFR modulates microRNA maturation in response to hypoxia through 
            phosphorylation of AGO2.
  JOURNAL   Nature. 2013 May 16;497(7449):383-7. doi: 10.1038/nature12080. Epub 
            2013 May 1.
  PUBMED    23636329
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12080"; journalName: "Nature"; date: "2013-05-16- 
            16"; volume: "497"; issue: "7449"; pages: "383-7"
COMMENT     Sequence Label: pMIR-192-Reporter
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     162..184
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     176..193
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     177..193
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
       61 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
      121 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
      181 aacgacggcc agtgccaagc tagcggccgc atacaaaaaa ccaacacaca gatccaatga
      241 aaataaaaga tcctttatta agcttctgac ctatgaattg acagccacta gtgcgcagct
      301 ttcatcactc gagcaatttg gactttccgc ccttcttggc ctttatgagg atctctctga
      361 tttttcttgc gtcgagtttt ccggtaagac ctttcggtac ttcgtccaca aacacaactc
      421 ctccgcgcaa ctttttcgcg gttgttactt gactggcgac gtaatccacg atctcttttt
      481 ccgtcatcgt ctttccgtgc tccaaaacaa caacggcggc gggaagttca ccggcgtcat
      541 cgtcgggaag acctgccacg cccgcgtcga agatgttggg gtgttgtaac aatatcgatt
      601 ccaattcagc gggggccacc tgatatcctt tgtatttaat taaagacttc aagcggtcaa
      661 ctatgaagaa gtgttcgtct tcgtcccagt aagctatgtc tccagaatgt agccatccat
      721 ccttgtcaat caaggcgttg gtcgcttccg gattgtttac ataaccggac ataatcatag
      781 gtcctctgac acataattcg cctctctgat taacgcccag cgttttcc
//
