LOCUS       sequence-133343-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet inducible, Gateway adapted ROSA26 targeting Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Melidoni AN, Dyson MR, Wormald S, McCafferty J
  TITLE     Selecting antagonistic antibodies that control differentiation 
            through inducible expression in embryonic stem cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Oct 29;110(44):17802-7. doi: 
            10.1073/pnas.1312062110. Epub 2013 Sep 30.
  PUBMED    24082130
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1312062110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-10-29- 29"; volume: "110"; issue: "44"; pages: "17802-7"
COMMENT     Sequence Label: pGATOR
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(108..232)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    379..460
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(416..435)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(625..648)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
       61 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
      121 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
      181 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatatcgg
      241 taccgataag cttgtcgacg atatctctag aggatcataa tcagccatac cacatttgta
      301 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
      361 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
      421 agcatcacaa atttcacaaa taaagcattt ttttcactgc ctcgagcttc tctcctgact
      481 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct
      541 agagcggccg ctagctcgag tcgatctgat atcatcgatg aattctaccg ggtaggggag
      601 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
      661 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
      721 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
      781 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
      841 ctcgtg
//
