LOCUS       sequence-133345-       23446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet inducible, Gateway adapted ROSA26 targeting Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 23446)
  AUTHORS   Melidoni AN, Dyson MR, Wormald S, McCafferty J
  TITLE     Selecting antagonistic antibodies that control differentiation 
            through inducible expression in embryonic stem cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Oct 29;110(44):17802-7. doi: 
            10.1073/pnas.1312062110. Epub 2013 Sep 30.
  PUBMED    24082130
REFERENCE   2  (bases 1 to 23446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1312062110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-10-29- 29"; volume: "110"; issue: "44"; pages: "17802-7"
COMMENT     Sequence Label: pGATOR
FEATURES             Location/Qualifiers
     source          1..23446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1956..3038
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    2586..3234
                     /label=HA-L
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    3040..3850
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     misc_feature    3364..4013
                     /label=HA-R
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     misc_feature    join(4744..4809,4810..4847)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     CDS             join(4915..5532,5533..5919)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the reverse tetracycline repressor 
                     rTetR with the C-terminal activation domain of herpes 
                     simplex virus VP16"
                     /label=rtTA
                     /note="In the Tet-On(R) system, rtTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLKGESWQDFLRNNAKSFRCALLSHRNGAKVHSDTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIE
                     GLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHAD
                     ALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(4985..5004)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    5930..6064
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5954..5973)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        6436..6750
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    6444..6462
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6480..6498
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6515..6533
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6551..6569
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6587..6605
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6622..6640
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6658..6676
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6723..6747
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    6776..6900
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6925..6930,6931..6948,6949..6955)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7009..7668
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7075..7094)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8010..8315
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8194..8213
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8356..8480)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    8627..8708
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        8838..9337
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8873..8896)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9270..9289
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9356..9955
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9356..9375)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9852..9872
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(20290..20309)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(20291..20309)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(20316..20333)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(20316..20332)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(20325..20347)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      20473..20928
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(20560..20579)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     20770..20791
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(21174..21192)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        21260..21364
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(21365..21433,21434..22225)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(21583..21602)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      22396..22984
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     22885..22904
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     23138..23155
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    23170..23310
                     /label=bom
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg
       61 tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt
      121 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg
      181 taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg
      241 atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg
      301 gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt
      361 aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac
      421 ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag
      481 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc
      541 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
      601 ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg
      661 cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt
      721 tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg
      781 ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac
      841 gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc
      901 atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccaatg atcgaagtta
      961 ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc
     1021 tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa
     1081 tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg
     1141 ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga
     1201 cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg
     1261 tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc
     1321 ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg
     1381 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga gttaggccca
     1441 acgcggcgcc acggcgtttc ctggccggga atggcccgta cccgtgaggt gggggtgggg
     1501 ggcagaaaag gcggagcgag cccgaggcgg ggagggggag ggccaggggc ggagggggcc
     1561 ggcactactg tgttggcgga ctggcgggac tagggctgcg tgagtctctg agcgcaggcg
     1621 ggcggcggcc gcccctcccc cggcggcggc agcggcggca gcggcggcag ctcactcagc
     1681 ccgctgcccg agcggaaacg ccactgaccg cacggggatt cccagtgccg gcgccagggg
     1741 cacgcgggac acgccccctc ccgccgcgcc attggcctct ccgcccaccg ccccacactt
     1801 attggccggt gcgccgccaa tcagcggagg ctgccggggc cgcctaaaga agaggctgtg
     1861 ctttggggct ccggctcctc agagagcctc ggctaggtag gggatcggga ctctggcggg
     1921 agggcggctt ggtgcgtttg cggggatggg cggccgcggc aggccctccg agcgtggtgg
     1981 agccgttctg tgagacagcc gggtacgagt cgtgacgctg gaaggggcaa gcgggtggtg
     2041 ggcaggaatg cggtccgccc tgcagcaacc ggagggggag ggagaaggga gcggaaaagt
     2101 ctccaccgga cgcggccatg gctcgggggg gggggggcag cggaggagcg cttccggccg
     2161 acgtctcgtc gctgattggc ttcttttcct cccgccgtgt gtgaaaacac aaatggcgtg
     2221 ttttggttgg cgtaaggcgc ctgtcagtta acggcagccg gagtgcgcag ccgccggcag
     2281 cctcgctctg cccactgggt ggggcgggag gtaggtgggg tgaggcgagc tggacgtgcg
     2341 ggcgcggtcg gcctctggcg gggcggggga ggggagggag ggtcagcgaa agtagctcgc
     2401 gcgcgagcgg ccgcccaccc tccccttcct ctgggggagt cgttttaccc gccgccggcc
     2461 gggcctcgtc gtctgattgg ctctcggggc ccagaaaact ggcccttgcc attggctcgt
     2521 gttcgtgcaa gttgagtcca tccgccggcc agcgggggcg gcgaggaggc gctcccaggt
     2581 tccggccctc ccctcggccc cgcgccgcag agtctggccg cgcgcccctg cgcaacgtgg
     2641 caggaagcgc gcgctggggg cggggacggg cagtagggct gagcggctgc ggggcgggtg
     2701 caagcacgtt tccgacttga gttgcctcaa gaggggcgtg ctgagccaga cctccatcgc
     2761 gcactccggg gagtggaggg aaggagcgag ggctcagttg ggctgttttg gaggcaggaa
     2821 gcacttgctc tcccaaagtc gctctgagtt gttatcagta agggagctgc agtggagtag
     2881 gcggggagaa ggccgcaccc ttctccggag gggggagggg agtgttgcaa tacctttctg
     2941 ggagttctct gctgcctcct ggcttctgag gaccgccctg ggcctgggag aatcccttcc
     3001 ccctcttccc tcgtgatctg caactccagt ctttctagaa gatgggcggg agtcttctgg
     3061 gcaggcttaa aggctaacct ggtgtgtggg cgttgtcctg caggggaatt gaacaggtgt
     3121 aaaattggag ggacaagact tcccacagat tttcggtttt gtcgggaagt tttttaatag
     3181 gggcaaataa ggaaaatggg aggataggta gtcatctggg gttttatgca gcaaaactac
     3241 aggttattat tgcttgtgat ccgcctcgga gtattttcca tcgaggtaga ttaaagacat
     3301 gctcacccga gttttatact ctcctgcttg agatccttac tacagtatga aattacagtg
     3361 tcgcgagtta gactatgtaa gcagaatttt aatcattttt aaagagccca gtacttcata
     3421 tccatttctc ccgctccttc tgcagcctta tcaaaaggta ttttagaaca ctcattttag
     3481 ccccattttc atttattata ctggcttatc caacccctag acagagcatt ggcattttcc
     3541 ctttcctgat cttagaagtc tgatgactca tgaaaccaga cagattagtt acatacacca
     3601 caaatcgagg ctgtagctgg ggcctcaaca ctgcagttct tttataactc cttagtacac
     3661 tttttgttga tcctttgcct tgatccttaa ttttcagtgt ctatcacctc tcccgtcagg
     3721 tggtgttcca catttgggcc tattctcagt ccagggagtt ttacaacaat agatgtattg
     3781 agaatccaac ctaaagctta actttccact cccatgaatg cctctctcct ttttctccat
     3841 ttataaactg agctattaac cattaatggt ttccaggtgg atgtctcctc ccccaatatt
     3901 acctgatgta tcttacatat tgccaggctg atattttaag acattaaaag gtatatttca
     3961 ttattgagcc acatggtatt gattactgct tactaaaatt ttgtcattgt acacatctgt
     4021 aaaaggtggt tccttttgga atgcaaagtt caggtgtttg ttgtctttcc tgacctaagg
     4081 tcttgtgagc ttgtattttt tctatttaag cagtgctttc tcttggactg gcttgactca
     4141 tggcattcta cacgttattg ctggtctaaa tgtgattttg ccaagcttct tcaggaccta
     4201 taattttgct tgacttgtag ccaaacacaa gtaaaatgat taagcaacaa atgtatttgt
     4261 gaagcttggt ttttaggttg ttgtgttgtg tgtgcttgtg ctctataata atactatcca
     4321 ggggctggag aggtggctcg gagttcaaga gcacagactg ctcttccaga agtcctgagt
     4381 tcaattccca gcaaccacat ggtggctcac aaccatctgt aatgggatct gatgccctct
     4441 tctggtgtgt ctgaagacca caagtgtatt cacattaaat aaataaatcc tccttcttct
     4501 tctttttttt ttttttaaag agaatactgt ctccagtaga atttactgaa gtaatgaaat
     4561 actttgtgtt tgttccaata tggtagccaa taatcaaatt actctttaag cactggaaat
     4621 gttaccaagg aactaatttt tatttgaagt gtaactgtgg acagaggagc cataactgca
     4681 gacttgtggg atacagaaga ccaatgcaga ctttaatgtc tttagcctta attaagggat
     4741 ctgtagggcg cagtagtcca gggtttcctt gatgatgtca tacttatcct gtcccttttt
     4801 tttccacagc tcgcggttga ggacaaactc ttcgcggtct ttccagtggg gatcgacggt
     4861 atcgtagagt cgaggccgct ctaggaattc accatgccaa agagacccag accctctaga
     4921 ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa
     4981 ggtttaacaa cccgtaaact cgcccagaag cttggtgtag agcagcctac actgtattgg
     5041 catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac
     5101 catactcact tttgcccttt aaaaggggaa agctggcaag attttttacg caataacgct
     5161 aaaagtttta gatgtgcttt actaagtcat cgcaatggag caaaagtaca ttcagataca
     5221 cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa
     5281 ggtttttcac tagagaacgc gttatatgca ctcagcgctg tggggcattt tactttaggt
     5341 tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact
     5401 gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag
     5461 ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt
     5521 gaaagtgggt ccgcgtacag ccgcgcgcgt acgaaaaaca attacgggtc taccatcgag
     5581 ggcctgctcg atctcccgga cgacgacgcc cccgaagagg cggggctggc ggctccgcgc
     5641 ctgtcctttc tccccgcggg acacacgcgc agactgtcga cggccccccc gaccgatgtc
     5701 agcctggggg acgagctcca cttagacggc gaggacgtgg cgatggcgca tgccgacgcg
     5761 ctagacgatt tcgatctgga catgttgggg gacggggatt ccccgggtcc gggatttacc
     5821 ccccacgact ccgcccccta cggcgctctg gatatggccg acttcgagtt tgagcagatg
     5881 tttaccgatg cccttggaat tgacgagtac ggtgggtagg gggcgcgagg atccagacat
     5941 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     6001 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     6061 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     6121 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc ctgcaagcct
     6181 cgtcgtcctg gccggaccac gctatctgtg caaggtcccc ggccccggac gcgcgctcca
     6241 tgagcagagc gcccgccgcc gaggcgaaga ctcgggcggc gccctgcccg tcccaccagg
     6301 tcaacaggcg gtaaccggcc tcttcatcgg gaatgcgcgc gaccttcagc atcgccggca
     6361 tgtccccctg gcggacggga agtatccagc tcgaccaagc ttggcgagat ttctaaggaa
     6421 gcttcgtctt cactcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact
     6481 ccctatcagt gatagagaac gatgtcgagt ttactcccta tcagtgatag agaacgtatg
     6541 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
     6601 agagaacgta tgtcgagttt atccctatca gtgatagaga acgtatgtcg agtttactcc
     6661 ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac ggtgggaggc ctatataagc
     6721 agagctcgtt tagtgaaccg tcagatcgcc tggaggatcc ccgggtaccg atatcacaag
     6781 tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta
     6841 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg
     6901 gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg
     6961 attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     7021 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     7081 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     7141 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     7201 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
     7261 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     7321 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     7381 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     7441 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     7501 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     7561 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
     7621 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaaac gcgtggatcc
     7681 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
     7741 atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta
     7801 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat
     7861 ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg
     7921 gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc
     7981 ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag
     8041 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac
     8101 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt
     8161 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg
     8221 tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca
     8281 aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc
     8341 agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
     8401 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
     8461 gctttcttgt acaaagtggt gatatcggta ccgataagct tgtcgacgat atctctagag
     8521 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     8581 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     8641 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8701 ttcactgcct cgagcttctc tcctgactac tcccagtcat agctgtccct cttctcttat
     8761 ggagatccct cgacctgcag cccaagctag agcggccgct agctcgagtc gatctgatat
     8821 catcgatgaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     8881 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     8941 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     9001 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     9061 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     9121 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     9181 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     9241 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     9301 cgcgctgttc tcctcttcct catctccggg cctttcgacc gatccagccg ccaccatgac
     9361 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac
     9421 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca
     9481 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     9541 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     9601 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     9661 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     9721 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     9781 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     9841 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     9901 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
     9961 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct ccgaccgaag
    10021 ccacccgggg cggccccgcc gaccccgcac ccgcccccga ggcccaccgc gggggacaca
    10081 ccgaacacgc cgaccctgct gaacacgcgg cgcagttcgg tgcccaggag cggatcgaaa
    10141 ttgatgatct attaaacaat aaagatgtcc actaaaatgg aagtttttcc tgtcatactt
    10201 tgttaagaag ggtgagaaca gagtacctac attttgaatg gaaggattgg agctacgggg
    10261 gtgggggtgg ggtgggatta gataaatgcc tgctctttac tgaaggctct ttactattgc
    10321 tttatgataa tgtttcatag ttggatatca taatttaaac aagcaaaacc aaattaaggg
    10381 ccagctcatt cctcccactc atgatctata gatcgacgcg tacgaattcg aatcgatcta
    10441 gctttactta acttttgtgc ttcatctata caaagctgaa agctaagtct gcagccatta
    10501 ctaaacatga aagcaagtaa tgataatttt ggatttcaaa aatgtagggc cagagtttag
    10561 ccagccagtg gtggtgcttg cctttatgcc tttaatccca gcactctgga ggcagagaca
    10621 ggcagatctc tgagtttgag cccagcctgg tctacacatc aagttctatc taggatagcc
    10681 aggaatacac acagaaaccc tgttggggag gggggctctg agatttcata aaattataat
    10741 tgaagcattc cctaatgagc cactatggat gtggctaaat ccgtctacct ttctgatgag
    10801 atttgggtat tattttttct gtctctgctg ttggttgggt cttttgacac tgtgggcttt
    10861 ctttaaagcc tccttcctgc catgtggtct cttgtttgct actaacttcc catggcttaa
    10921 atggcatggc tttttgcctt ctaagggcag ctgctgagat ttgcagcctg atttccaggg
    10981 tggggttggg aaatctttca aacactaaaa ttgtccttta attttttttt taaaaaatgg
    11041 gttatataat aaacctcata aaatagttat gaggagtgag gtggactaat attaaatgag
    11101 tccctcccct ataaaagagc tattaaggct ttttgtctta tacttaactt tttttttaaa
    11161 tgtggtatct ttagaaccaa gggtcttaga gttttagtat acagaaactg ttgcatcgct
    11221 taatcagatt ttctagtttc aaatccagag aatccaaatt cttcacagcc aaagtcaaat
    11281 taagaatttc tgacttttaa tgttaatttg cttactgtga atataaaaat gatagctttt
    11341 cctgaggcag ggtctcacta tgtatctctg cctgatctgc aacaagatat gtagactaaa
    11401 gttctgcctg cttttgtctc ctgaatacta aggttaaaat gtagtaatac ttttggaact
    11461 tgcaggtcag attcttttat aggggacaca ctaagggagc ttgggtgata gttggtaaaa
    11521 tgtgtttcaa gtgatgaaaa cttgaattat tatcaccgca acctactttt taaaaaaaaa
    11581 agccaggcct gttagagcat gcttaaggga tccctaggac ttgctgagca cacaagagta
    11641 gttacttggc aggctcctgg tgagagcata tttcaaaaaa caaggcagac aaccaagaaa
    11701 ctacagttaa ggttacctgt ctttaaacca tctgcatata cacagggata ttaaaatatt
    11761 ccaaataata tttcattcaa gttttccccc atcaaattgg gacatggatt tctccggtga
    11821 ataggcagag ttggaaacta aacaaatgtt ggttttgtga tttgtgaaat tgttttcaag
    11881 tgatagttaa agcccatgag atacagaaca aagctgctat ttcgaggtct cttggtttat
    11941 actcagaagc acttctttgg gtttccctgc actatcctga tcatgtgcta ggcctacctt
    12001 aggctgattg ttgttcaaat aaacttaagt ttcctgtcag gtgatgtcat atgatttcat
    12061 atatcaaggc aaaacatgtt atatatgtta aacatttgta cttaatgtga aagttaggtc
    12121 tttgtgggtt tgatttttaa ttttcaaaac ctgagctaaa taagtcattt ttacatgtct
    12181 tacatttggt ggaattgtat aattgtggtt tgcaggcaag actctctgac ctagtaaccc
    12241 tacctataga gcactttgct gggtcacaag tctaggagtc aagcatttca ccttgaagtt
    12301 gagacgtttt gttagtgtat actagtttat atgttggagg acatgtttat ccagaagata
    12361 ttcaggacta tttttgactg ggctaaggaa ttgattctga ttagcactgt tagtgagcat
    12421 tgagtggcct ttaggcttga attggagtca cttgtatatc tcaaataatg ctggcctttt
    12481 ttaaaaagcc cttgttcttt atcaccctgt tttctacata atttttgttc aaagaaatac
    12541 ttgtttggat ctccttttga caacaatagc atgttttcaa gccatatttt ttttcctttt
    12601 tttttttttt tttggttttt cgagacaggg tttctctgta tagccctggc tgtcctggaa
    12661 ctcactttgt agaccaggct ggcctcgaac tcagaaatcc gcctgcctct gcctcctgag
    12721 tgccgggatt aaaggcgtgc accaccacgc ctggctaagt tggatatttt gttatataac
    12781 tataaccaat actaactcca ctgggtggat ttttaattca gtcagtagtc ttaagtggtc
    12841 tttattggcc cttcattaaa atctactgtt cactctaaca gaggctgttg gtactagtgg
    12901 cacttaagca acttcctacg gatatactag cagattaagg gtcagggata gaaactagtc
    12961 tagcgttttg tatacctacc agctttatac taccttgttc tgatagaaat atttcaggac
    13021 atctagagtg tactataagg ttgatggtaa gcttataagg aacttgaaag tggagtaact
    13081 actccatttc tctgagggga gaattaaaat ttttgaccaa gtgttgttga gccactgaga
    13141 atggtctcag aacataactt cttaaggaac cttcccagat tgccctcaac actgcaccac
    13201 atttggtcct gcttgaacat tgccatggct cttaaagtct taattaagaa tattaattgt
    13261 gtaattattg tttttcctcc tttagatcat tccttgagga caggacagtg cttgtttaag
    13321 gctatatttc tgctgtctga gcagcaacag gtcttcgaga tcaacatgat gttcataatc
    13381 ccaagatgtt gccatttatg ttctcagaag caagcagagg catgatggtc agtgacagta
    13441 atgtcactgt gttaaatgtt gctatgcagt ttggattttt ctaatgtagt gtaggtagaa
    13501 catatgtgtt ctgtatgaat taaactctta agttacacct tgtataatcc atgcaatgtg
    13561 ttatgcaatt accattttaa gtattgtagc tttctttgta tgtgaggata aaggtgtttg
    13621 tcataaaatg ttttgaacat ttccccaaag ttccaaatta taaaaccaca acgttagaac
    13681 ttatttatga acaatggttg tagtttcatg cttttaaaat gcttaattat tcaattaaca
    13741 ccgtttgtgt tataatatat ataaaactga catgtagaag tgtttgtcca gaacatttct
    13801 taaatgtata ctgtctttag agagtttaat atagcatgtc ttttgcaaca tactaacttt
    13861 tgtgttggtg cgagcaatat tgtgtagtca ttttgaaagg agtcatttca atgagtgtca
    13921 gattgttttg aatgttattg aacattttaa atgcagactt gttcgtgttt tagaaagcaa
    13981 aactgtcaga agctttgaac tagaaattaa aaagctgaag tatttcagaa gggaaataag
    14041 ctacttgctg tattagttga aggaaagtgt aatagcttag aaaatttaaa accatatagt
    14101 tgtcattgct gaatatctgg cagatgaaaa gaaatactca gtggttcttt tgagcaatat
    14161 aacagcttgt tatattaaaa attttcccca cagatataaa ctctaatcta taactcataa
    14221 atgttacaaa tggatgaagc ttacaaatgt ggcttgactt gtcactgtgc ttgttttagt
    14281 tatgtgaaag tttggcaata aacctatgtc ctaaatagtc aaactgtgga atgacttttt
    14341 aatctattgg tttgtctaga acagttatgt tgccatttgc cctaatggtg aaagaaaaag
    14401 tggggagtgc cttggcactg ttcatttgtg gtgtgaacca aagagggggg catgcactta
    14461 cacttcaaac atccttttga aagactgaca agtttgggtc ttcacagttg gaattgggca
    14521 tcccttttgt cagggaggga gggagggagg gaggctggct tgttatgctg acaagtgtga
    14581 ttaaattcaa actttgaggt aagttggagg aacttgtaca ttgttaggag tgtgacaatt
    14641 tggactctta atgatttggt catacaaaat gaacctagac caacttctgg aagatgtata
    14701 taataactcc atgttacatt gatttcacct gactaatact tatcccttat caattaaata
    14761 cagaagatgc cagccatctg ggccttttaa cccagaaatt tagtttcaaa ctcctaggtt
    14821 agtgttctca ctgagctaca tcctgatcta gtcctgaaaa taggaccacc atcaccccca
    14881 aaaaaatctc aaataagatt tatgctagtg tttcaaaatt ttaggaatag gtaagattag
    14941 aaagttttaa attttgagaa atggcttctc tagaaagatg tacatagtga acactgaatg
    15001 gctcctaaag agcctagaaa actggtactg agcacacagg actgagaggt ctttcttgaa
    15061 aagcatgtat tgctttacgt gggtcacaga aggcaggcag gaagaacttg ggctgaaact
    15121 ggtgtcttaa gtggctaaca tcttcacaac tgatgagcaa gaactttatc ctgatgcaaa
    15181 aaccatccaa acaaactaag tgaaaggtgg caatggatcc caggctgctc tagaggagga
    15241 cttgacttct catcccatca cccacaccag atagctcata gactgccaat taacaccagc
    15301 ttctagcctc cacaggcacc tgcactggta cacataattt cacacaaaca cagtaagaag
    15361 ccttccacct ggcatggtat tgcttatctt tagttcccaa cacttgggag gcagaggcca
    15421 gccagggcta tgtgacaaaa accttgtcta gaggagaaac ttcatagctt atttcctatt
    15481 cacgtaacca ggttagcaaa atttaccagc cagagatgaa gctaacagtg tccactatat
    15541 ttgtagtgtt ttaagtcaat tttttaaata tacttaatag aattaaagct atggtgaacc
    15601 aagtacaaac ctggtgtatt aacttgagaa cttagcataa aaagtagttc atttgttcag
    15661 taaatattaa atgcttactg gcaaagatta tgtcaggaac ttggtaaatg gtgatgaaac
    15721 aatcatagtt gtacatcttg gttctgtgat caccttggtt tgaggtaaaa gtggttcctt
    15781 tgatcaagga tggaatttta agtttatatt caatcaataa tgtattattt tgtgattgca
    15841 aaattgccta tctagggtat aaaaccttta aaaatttcat aataccagtt cattctccag
    15901 ttactaattc caaaaagcca ctgactatgg tgccaatgtg gattctgttc tcaaaggaag
    15961 gattgtctgt gccctttatt ctaatagaaa catcacactg aaaatctaag ctgaaagaag
    16021 ccagactttc ctaaataaat aactttccat aaagctcaaa caaggattac ttttaggagg
    16081 cactgttaag gaactgataa gtaatgaggt tacttatata atgatagtcc cacaagacta
    16141 tctgaggaaa aatcagtaca actcgaaaac agaacaacca gctaggcagg aataacaggg
    16201 ctcccaagtc aggaggtcta tccaacaccc ttttctgttg agggccccag acctacatat
    16261 tgtatacaaa cagggaggtg ggtgatttta actctcctga ggtaccttgg taaatctttg
    16321 tcctgagtaa gcagtacagt gtacagttta cattttcatt taaagataca ttagctccct
    16381 ctacccccta agactgacag gcactttggg ggtggggagg gctttggaaa ataacgcttc
    16441 catacactaa aagagaaatt tctttaatta ggcttgttgg ttccatacat ctactggtgt
    16501 ttctactact tagtaatatt ataatagtca cacaagcatc tttgctctgt ttaggttgta
    16561 tatttatttt aaggcagatg ataaaactgt agatcttaag ggatgcttct gcttctgaga
    16621 tgatacaaag aatttagacc ataaaacagt aggttgcaca agcaatagaa tatggcctaa
    16681 agtgttctga cacttagaag ccaagcagtg taggcttctt aagaaatacc attacaatca
    16741 ccttgctaga aatcaagcat tctggagtgg tcaagcagtg taacctgtac tgtaagttac
    16801 ttttctgcta tttttctccc aaagcaagtt ctttatgctg atatttccag tgttaggaac
    16861 tacaaatatt aataagttgt cttcactctt ttctttacca aggagggtct cttccttcat
    16921 cttgatctga aggatgaaca aaggcttgag cagtgcgctt tagaagataa actgcagcat
    16981 gaaggccccc gatgttcacc cagactacat ggacctttcg ccacacatgt cccattccag
    17041 ataaggcctg gcacacacaa aaaacataag tcattaggct accagtctga ttctaaaaca
    17101 acctaaaatc ttcccactta aatgctatgg gtggtgggtt ggaaagttga ctcagaaaat
    17161 cacttgctgt ttttagagag gatctgggtt cagtttctga tacattgtgg cttacaacta
    17221 taactccagt tctagggggt ccatccaaca tcctcttctg ttgagggcac caaataaatg
    17281 tattgtgtac aaacagggag gtgagtgatt taactctcgt gtatagtacc ttggtaaaac
    17341 atttcttgtc ctgagtaagc agtacagctc tgcctgtccc tggtctacag acacggctca
    17401 tttcccgaag gcaagctgga tagagattcc aatttctctt cttggatccc atcctataaa
    17461 agaaggtcaa gtttaatcta ttgcaaaagg taaataggta gtttcttaca tgagacaaga
    17521 acaaatctta ggtgtgaagc agtcatcttt tacaggccag agcctctatt ctatgccaat
    17581 gaaggaaact gttagtccag tgttatagag ttagtccagt gtatagtttt ctatcagaac
    17641 actttttttt taaacaactg caacttagct tattgaagac aaaccacgag tagaaatctg
    17701 tccaagaagc aagtgcttct cagcctacaa tgtggaatag gaccatgtaa tggtacagtg
    17761 agtgaaatga attatggcat gtttttctga ctgagaagac agtacaataa aaggtaaact
    17821 catggtattt atttaaaaag aatccaattt ctaccttttt ccaaatggca tatctgttac
    17881 aataatatcc acagaagcag ttctcagtgg gaggttgcag atatcccact gaacagcatc
    17941 aatgggcaaa ccccaggttg tttttctgtg gagacaaagg taagatattt caatatattt
    18001 tcccaagcta atgagatggc tcagcaaata atggtactgg ccattaagtc tcatgacctg
    18061 agcttgatcc tcagggacca tgtggtacaa ggagagacct aaatccttca gttggacttc
    18121 aatcttctac cctcatgtcc acacacaaat aaatacaata aaaaacattc tgcagtctga
    18181 atttctaaag gttgtttttc taaaaagaaa tgttaaagta acataggaag aaatatgtcc
    18241 ataactgaaa tacaagtttt ttaaatggtt aagactggtt ttcaaaggat gtatggttaa
    18301 gaaaatacca gggaaaatga gcttacatgt aaaaaagtgt ctaaaaggcc agagaaatga
    18361 cccagctggc aaaggtgtct gccctaagcc agacaaaagg aatttgattc acaggaagaa
    18421 gagacccaac tctcactagt tatcctctga cttccacacc atgacacagc tccatggcac
    18481 tctcaggccc ccacacatat acagatataa acagaaacct aatccaccag ccttcagaag
    18541 caaagcaatt ggaggattta aacaggccat ggctactaat agagataact ggtagtttaa
    18601 aagttatggt aatgactttc atgcttcttt caactcatat tgttctaaat aattaatttg
    18661 gtttttcaag gcagggtttc tctgtgtagt tctggctgtc ctggaactca ctctgtagac
    18721 caggctggcc ttgaactcag atccatctgc ctctggaata agggcacgtg cgtgcctttt
    18781 ctacataaca aaacctatac tataacaaaa cctataccat actgtaccgt tttgggaaaa
    18841 gacaaaaaat aatgaacaaa aaaggagaaa taacattcca ataaagtatg gaaatggtag
    18901 ttaaattaat tacaaatgtt tttcagtaaa ttagatgtga cttctcatac tgttcatttg
    18961 gctataatga taccacaaag cactgggggt gaataataat tccaagtcag tagggagaga
    19021 gacttgaaaa gatgcaatgc aatcattgaa gttaaactta cccatcttta atctggctct
    19081 tagtcaatag agatgagatg ttatttgctg ctctgttcac tgccagtggg ttattgtccc
    19141 cagcaatatg gtaacagtga gaccactcag tagcccccta tgagacagga gtgttggtta
    19201 aacatgccac aagagaaaag ggaaaagtca ctatggccaa ctctcagtaa catggcaatc
    19261 cgtgccattc atttccttgc cagaaatgtc ttccctgttc ttctgcctac tgaactttca
    19321 cccactagaa atgtggctcc aatgtcatcc actatgacat caatgtcagc gctagaagca
    19381 ctttgcacac ctctgttgct gacttagccc atttgaaact atgtctatca cctctgccta
    19441 agtaatccag caattccttc cagataggtc tagtattttc tttctacccg gcaatggctg
    19501 gcacatataa gggactgcat tctcttaatt aacatgattt ttaaaaattt acctaactaa
    19561 ccagacctat gctgtcttgg attgtgagtt tagcttttaa aagataacca catttctgaa
    19621 aagattttgt agttaagtaa aaaatgtcag cagcatataa atgccaagca gtgacatatt
    19681 tgtgaaacaa agtgacatac tatggtactg tttctgccac aaagacatgc atgcatgaat
    19741 acagcacttc ataactattt tgcccagacc ctttaggatg atgacattct ggtctattat
    19801 tctataatga cagcaaacaa gaggaatgtt caagtatgcc ataaactggg tgtgcaggga
    19861 tacaggaaga actcacaggt gtgatctgtg cttgccaacc tttttatttt ttttaattat
    19921 tattttaatg tttaatccag gatggatttc ctaccttggg ctacatttac agtacttcta
    19981 aatctcagtg tcttctagga caggtattag ataatttaat gggcacatct gtacacagac
    20041 ccttcagttt ccatcatgac tcagctgaag tttccacgta ggtactctct ctcatgccta
    20101 cttttacgtg gaaatggctt aatctggttt tcccacccca ccccttttat tctgatgtct
    20161 actttgattg tgtgtttctt catcatggtc agtttacctg ccatttcgca gataagtcat
    20221 ggtttaatga tggagaacta cagtttggtt tccagaatta attcgagctc ggccgacttg
    20281 gccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt
    20341 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
    20401 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
    20461 aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
    20521 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
    20581 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
    20641 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
    20701 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
    20761 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
    20821 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
    20881 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta
    20941 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
    21001 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
    21061 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
    21121 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
    21181 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
    21241 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    21301 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    21361 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
    21421 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
    21481 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
    21541 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
    21601 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    21661 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    21721 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    21781 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    21841 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    21901 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    21961 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    22021 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    22081 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    22141 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    22201 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    22261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    22321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    22381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    22441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    22501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
    22561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    22621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    22681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    22741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    22801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    22861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    22921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    22981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    23041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    23101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    23161 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
    23221 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
    23281 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
    23341 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
    23401 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcga
//
