LOCUS       sequence-133346-         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet inducible, Gateway adapted ROSA26 targeting Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Melidoni AN, Dyson MR, Wormald S, McCafferty J
  TITLE     Selecting antagonistic antibodies that control differentiation 
            through inducible expression in embryonic stem cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Oct 29;110(44):17802-7. doi: 
            10.1073/pnas.1312062110. Epub 2013 Sep 30.
  PUBMED    24082130
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1312062110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-10-29- 29"; volume: "110"; issue: "44"; pages: "17802-7"
COMMENT     Sequence Label: pGATOR
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(81..87,88..105,106..111))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(136..260)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(286..600)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(289..313)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    360..378
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    396..414
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    431..449
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    467..485
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    503..521
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    538..556
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    574..592
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg
       61 atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggt
      121 gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
      181 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
      241 gcttttttgt acaaacttgt gatatcggta cccggggatc ctccaggcga tctgacggtt
      301 cactaaacga gctctgctta tataggcctc ccaccgtaca cgcctacctc gacatacgtt
      361 ctctatcact gatagggagt aaactcgaca tacgttctct atcactgata gggataaact
      421 cgacatacgt tctctatcac tgatagggag taaactcgac atacgttctc tatcactgat
      481 agggagtaaa ctcgacatac gttctctatc actgataggg agtaaactcg acatcgttct
      541 ctatcactga tagggagtaa actcgacata cgttctctat cactgatagg gagtaaactc
      601 gagtgaagac gaagcttcct tagaaatctc gccaagcttg gtcgagctgg atacttcccg
      661 tccgc
//
