LOCUS       sequence-133350-        6678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mammalian expression of human SRF with an HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Johansen FE, Prywes R
  TITLE     Identification of transcriptional activation and inhibitory domains 
            in serum response factor (SRF) by using GAL4-SRF constructs.
  JOURNAL   Mol Cell Biol. 1993 Aug . 13(8):4640-7.
  PUBMED    8336707
REFERENCE   2  (bases 1 to 6678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1993 Aug . 13(8):4640-7."
COMMENT     Sequence Label: pCGN-SRF
FEATURES             Location/Qualifiers
     source          1..6678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           598..637
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             667..693
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             673..699
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     intron          2377..2949
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3081..3100)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3146..3201
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3200..3219)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      3555..3690
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3796..3813)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3967..4555)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4047..4066)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4726..5517,5518..5586))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5349..5368
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5587..5691)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5759..5777
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5815..5837)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5937..5956
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6023..6478)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6160..6181)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6372..6391
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6604..6626
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6618..6635
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6619..6635
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6642..6661
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6642..6660
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgggggat
      601 cttggtggcg tgaaactccc gcacctcttc ggccagcgcc ttgtagaagc gcgtatggct
      661 tctagctatc cttatgacgt gcctgactat gccagcctgg gaggaccttc tagagctatg
      721 ttaccgaccc aagctggggc cgcggcggct ctgggccggg gctcggccct ggggggcagc
      781 ctgaaccgga ccccgacggg gcggccgggc ggcggcggcg ggacacgcgg ggctaacggg
      841 ggccgggtcc ccgggaatgg cgcggggctc gggcccggcc gcctggagcg ggaggctgcg
      901 gcagcggcgg caaccacccc ggcgcccacc gcgggggccc tctacagcgg cagcgagggc
      961 gactcggagt cgggcgagga ggaggagctg ggcgccgagc ggcgcggcct gaagcggagc
     1021 ctgagcgaga tggagatcgg tatggtggtc ggtgggcccg aggcgtcggc agcggccacc
     1081 gggggctacg ggccggtgag cggcgcggtg agcggggcca agccgggtaa gaagacccgg
     1141 ggccgcgtga agatcaagat ggagttcatc gacaacaagc tgcggcgcta cacgaccttc
     1201 agcaagagga agacgggcat catgaagaag gcctatgagc tgtccacgct gacagggaca
     1261 caggtgctgt tgctggtggc cagtgagaca ggccatgtgt atacctttgc cacccgaaaa
     1321 ctgcagccca tgatcaccag tgagaccggc aaggcactga ttcagacctg cctcaactcg
     1381 ccagactctc caccccgttc agaccccaca acagaccaga gaatgagtgc cactggcttt
     1441 gaagagacag atctcaccta ccaggtgtcg gagtctgaca gcagtgggga gaccaaggac
     1501 acactgaagc cggcgttcac agtcaccaac ctgccgggta caacctccac catccaaaca
     1561 gcacctagca cctctaccac catgcaagtc agcagcggcc cctcctttcc catcaccaac
     1621 tacctggcac cagtgtctgc tagtgtcagc cccagtgctg tcagcagtgc caatgggact
     1681 gtgctgaaga gtacaggcag cggccctgtc tcctctgggg gccttatgca gctgcctacc
     1741 agcttcaccc tcatgcctgg tggggcagtg gcccagcagg tcccagtgca ggccattcaa
     1801 gtgcaccagg ccccacagca agcgtctccc tcccgtgaca gcagcacaga cctcacgcag
     1861 acctcctcca gcgggacagt gacgctgccc gccaccatca tgacgtcatc cgtgcccaca
     1921 actgtgggtg gccacatgat gtaccctagc ccgcatgcgg tgatgtatgc ccccacctcg
     1981 ggcctgggtg atggcagcct caccgtgctg aatgccttct cccaggcacc atccaccatg
     2041 caggtgtcac acagccaggt ccaggagcca ggtggcgtcc cccaggtgtt cctgacagca
     2101 tcatctggga cagtgcagat ccctgtttca gcagttcagc tccaccagat ggctgtgata
     2161 gggcagcagg ccgggagcag cagcaacctc accgagctac aggtggtgaa cctggacacc
     2221 gcccacagca ccaagagtga atgatccgcc cgccgccctg gacagatggc ccaagggatg
     2281 gcaccactta tttattgttg ccttttcacg ttttctttac acacacgttg actctagaat
     2341 tcgagctcgg tacccgggga tcctgagaac ttcagggtga gtttggggac ccttgattgt
     2401 tctttctttt tcgctattgt aaaattcatg ttatatggag ggggcaaagt tttcagggtg
     2461 ttgtttagaa tgggaagatg tcccttgtat caccatggac cctcatgata attttgtttc
     2521 tttcactttc tactctgttg acaaccattg tctcctctta ttttcttttc attttctgta
     2581 actttttcgt taaactttag cttgcatttg taacgaattt ttaaattcac ttttgtttat
     2641 ttgtcagatt gtaagtactt tctctaatca cttttttttc aaggcaatca gggtatatta
     2701 tattgtactt cagcacagtt ttagagaaca attgttataa ttaaatgata aggtagaata
     2761 tttctgcata taaattctgg ctggcgtgga aatattctta ttggtagaaa caactacacc
     2821 ctggtcatca tcctgccttt ctctttatgg ttacaatgat atacactgtt tgagatgagg
     2881 ataaaatact ctgagtccaa accgggcccc tctgctaacc atgttcatgc cttcttctct
     2941 ttcctacagc tcctgggcaa cgtgctggtt gttgtgctgt ctcatcattt tggcaaagaa
     3001 ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg
     3061 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag
     3121 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     3181 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
     3241 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac
     3301 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta
     3361 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt
     3421 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc
     3481 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgaggagctt
     3541 tttgcaaaag cctaggcctc caaaaaagcc tcttcactac ttctggaata gctcagaggc
     3601 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     3661 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
     3721 tgactaattg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3781 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3841 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3901 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3961 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4021 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4081 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4141 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4201 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4261 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4321 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4381 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4441 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4501 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4561 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4621 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4681 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4741 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4801 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4861 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     4921 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4981 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     5041 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     5101 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     5161 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     5221 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     5281 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     5341 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5401 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5461 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5521 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5581 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5641 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5701 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     5761 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     5821 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     5881 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     5941 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     6001 aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta
     6061 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     6121 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     6181 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     6241 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     6301 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     6361 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     6421 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     6481 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     6541 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     6601 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
     6661 gcgaattcga gctcgccc
//
