LOCUS       sequence-133351-       11042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11042)
  AUTHORS   Schroeter EH, Kisslinger JA, Kopan R
  TITLE     Notch-1 signalling requires ligand-induced proteolytic release of 
            intracellular domain.
  JOURNAL   Nature. 1998 May 28;393(6683):382-6.
  PUBMED    9620803
REFERENCE   2  (bases 1 to 11042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "1998-05-28"; volume: "393(6683)"; pages: "382-6"
COMMENT     Sequence Label: pCS2 Notch1 Full Length-6MT
FEATURES             Location/Qualifiers
     source          1..11042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(21..1005)
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     rep_origin      1076..1531
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1163..1182)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1373..1394
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1557..1661
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1662..1730,1731..2522)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1880..1899)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2693..3281
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3182..3201
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3435..3452
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3569..3590
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3605..3610,3611..3628,3629..3635)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3643..3659
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3648..3670
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3667..3683
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3667..3683
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3702..3722
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3704..3722
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    3835..3969
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3872..3891)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3926..3945
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(4019..4048)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(4082..4111)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(4121..4150)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(4160..4189)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(4199..4228)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(4238..4267)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4281..4297)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4282..4298)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     regulatory      9018..9027
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      10287..10296
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      10619..10628
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(11010..11028)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(11011..11028)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cgtccccagg cagaatggcg gttccctaaa cgagcattgc ttatatagac ctcccattag
       61 gcacgcctac cgcccattta cgtcaatgga acgcccattt gcgtcattgc ccctccccat
      121 tgacgtcaat ggggatgtac ttggcagcca tcgcgggcca tttaccgcca ttgacgtcaa
      181 tgggagtact gccaatgtac cctggcgtac ttccaatagt aatgtacttg ccaagttact
      241 attaatagat attgatgtac tgccaagtgg gccatttacc gtcattgacg tcaatagggg
      301 gcgtgagaac ggatatgaat gggcaatgag ccatcccatt gacgtcaatg gtgggtggtc
      361 ctattgacgt caatgggcat tgagccaggc gggccattta ccgtaattga cgtcaatggg
      421 ggaggcgcca tatacgtcaa taggaccgcc catatgacgt caataggaaa gaccatatat
      481 agagaccatt gacgtcaatg ggggagtggc tatgggcggt attaggaagc cccatatatg
      541 gtatatggga ccgcccattg ggaggggcta tctacgtcaa taggaaaacc catatatgga
      601 atactatatg gcatagggcc aatacatagt attgaacctg gccaatagcc atattggcat
      661 agggccatat tggatattgc ctatatattg atcctggcat atagccaata tggccgccat
      721 tattggcacc atgccaattc aatatggcgg acttggcaca gggccaagac ccctcctcaa
      781 acttggcacc gtgccaagta gacccctccc cagttggcac agtgccaggt ccgccatatt
      841 gaattggcat ggtgccaagt ccgccatatt gaattggctg gggtccagat ccaccatatt
      901 gaattggcac gagtccatat acgccatatt gaattggcac aggtccagat ccaccatatt
      961 gaattggcat gagtccatat acgccatatt gaattggcgg tcgactggcg taatagcgaa
     1021 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     1081 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     1141 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     1201 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     1261 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     1321 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     1381 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     1441 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     1501 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     1561 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1621 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     1681 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1741 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1801 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1861 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     1921 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     1981 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2041 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     2101 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     2161 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     2221 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     2281 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2341 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2401 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     2461 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2521 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2581 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2641 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2701 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2761 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2821 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2881 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2941 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3001 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3061 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3121 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3181 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3241 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3301 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3361 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3421 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     3481 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     3541 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     3601 aggctttaca ctttatgctc ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     3661 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     3721 gggaacaaaa gctgggtacc gggcccaatg cattggcgcc gcggccgcga attaaaaaac
     3781 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     3841 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3901 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     3961 gtctggatct acgtaatacg actcactata gttctagagg ctcgagaggc cttgaattca
     4021 agtcctcttc agaaatgagc ttttgctcca tggtgaggtc gcccaagctc tccatttcat
     4081 tcaagtcctc ttcagaaatg agcttttgct ccatttcatt caagtcctct tcagaaatga
     4141 gcttttgctc catttcattc aagtcctctt cagaaatgag cttttgctcc atttcattca
     4201 agtcctcttc agaaatgagc ttttgctcca tttcattcaa gtcctcttca gaaatgagct
     4261 tttgctccat agctttaaat cgataccgtc gacctcgatg acgcgtcatc gagctgtcca
     4321 acaggcagcc cttgccatcc tgggacttct tcctccgtgc cttgaggtcc ttagcttcct
     4381 tgctaccaca agccagccct ttggtgctgg gcttgcgggc cttcttgccc tgtgtggcag
     4441 acttgagatt gcccaggtag ccattgggcg agcagagtgt gggagacaga gtgggtgtgc
     4501 cacccagggc agtgccatgc agctgtgggc tgcgcaccag gttgtactca tccaaaagcc
     4561 gcacgatatc gtggtgcata cgctcctgtg cgatgtcccg cggcaatcgg tccatgtgat
     4621 ccgtgatgtc ccggttggca aagtggtcca gcaacacttt ggcagtctca tagctgccct
     4681 cacgggcggc caggaacagg ggagtctcct ccttgttgtt ctgcatgtcc ttgttggctc
     4741 cgttcttcag gagcacaaca gcagcatcca cattgttcac cgcggccgcc caatgcaaag
     4801 ccgacttgcc taggtcatcc acggcattga cgtcagcatg tgagttgatg aggtcctcca
     4861 gcatgccctc cacggccagg cgcgcagcca ggatcagtgg agttgtgcca tcatgcattc
     4921 gggcatccag atctgtggcc ctgttccgga gcaggatctg gaagacaccc tgagcatctg
     4981 cagaaactgc tgcatgtaac ggagtacggc ccatgttgtc ctggatgttg gcatctgcac
     5041 tggcctccag caagcgcttt gcagcatctg aacgagagta tcgggcagcc aagtgcaagg
     5101 cggtctcccc ggtgcggtct gtctggttgt gcaagctggc gccctggtag atgaagtcag
     5161 agatgacagc aggtgcatct tcttcttctt cactgttgcc tgtctcaagg ccccctccac
     5221 tgcaggaggc aatcatgagg ggtgtgaagc catctggtcc tcgaacattg acatccatgc
     5281 agtcagcatc cacctccccc tgaggcggtg ttggggccat ggcagacatg cgcaggtcag
     5341 cagcgtccag gtgctgctgg gtccactgtc tgtggtcagt ctgatcactc aggtcaggga
     5401 gaactactgg ctcctcaaac cggaacttct tggtctccag gtcttcgtct ccccactcgt
     5461 tctgattgtc gtccatcaga gcaccatctg aggcattctt caggggcttg aggccgactg
     5521 agtcctcgcc gaggggctct ctccgcttct tcttgctggc ctctgacact ttgaaaccct
     5581 cagggaacca gagctggcca tgctgccgcc ggcgcttgcg ggacagcagc accccacagc
     5641 ccacaaagaa caggagcacg aaggcggccg ctgccacgta catgaggtgc agctgcgagg
     5701 gcagcggagg ctccaccggc tcactcttca cggcctcaat cttgtaagga atattgaggc
     5761 tgccaagtga cgcaagagca cctaggaagg cagccacatc ggtggcactc tggaagcact
     5821 gcgaggatga ctgcacacat tgccggttgt cgatctccag gtagacaatg gagccacgga
     5881 tgtccatggg gtccagctcc ctgcgctggc gcccaccact ggtaccagga agcagtgaag
     5941 aggtggccca acccactgta gagcgcttga ttgggtgctt gcgcagctct tcctcgtggc
     6001 catagtacgg gaagatcatc tgctggcctt gcgcatcacg cttgaagacc acgttggtgt
     6061 gcagcacgtg gctgagctcc cgcagaaagt ggaaggagtt gttccgtagc tggtcgggtg
     6121 gaagcagcac caccagcacc agggtgccgg ctgccagccg ctcgggtaca tgctcagcac
     6181 agtctaggcc atcccactca cattcggcac tgttacagcc ctggtcgcag tggccatcac
     6241 tgaagtggtc cttgcagtac tggtcataca gggggttgca ctgtccctcg gtgagctggc
     6301 agtcgaagcc atcaaagagg cagccggccg agttgcactg gctgtcacag tggccgtcgc
     6361 taaaatactt ccagcactgt agagactgcg tgcagttctt ccaggggtca ttgaagttga
     6421 gggagcagtc gccaccatcc cagccacatg cgtgattatt acactgcagg ttgcagacct
     6481 tattgcctgc atccacctgg cactcaggca gctcacaggc ctcctcaatc tgcggtgggg
     6541 gaatgtcgcg cccagcgcca cctgtgaagc tgtagtccag gatgtggcac agtagcccgt
     6601 tgaatttggc agggcataga cagcggtaga aagggttctc ggatgtgggc tcacaggtgc
     6661 cctgattgta gcaggggttg ctacccacac aggggctgct ggctgggaac tggcactcag
     6721 ggccggtgaa ggatcccagg cataggcagg tgggactacg tgggcccgag atgcatgtac
     6781 caccgttgag gcagcgtaag ctgccacaag tgcgggcatc attctcacat gtggcaccct
     6841 cgaagcccgc agggcaccta cagatgaatc cacgggcggt gttggaggcc acggcacaga
     6901 cacccccatt cttgcaaggt ttgcccctgc agccattgat gactgactca cagcggcgtc
     6961 cagtgtggcc agcccggcac tcgcagtgga agtcattaac acgctgcaca cagttctggg
     7021 tgccacgtgg gtcacagggg ttggagagac attcattgac atcaccctca caccgctccc
     7081 cgacgaagcc tggtgggcag gtgcaggtat agccacccac ctggtccaca caggtgccat
     7141 tgttgaagca cttggggctt cgggaggcag ggtcaagggg gggatggcag tcatcaacat
     7201 tgatctcaca gtgtacaccc tgtgtccccc gggggcagga acacttgtag gagttggtca
     7261 gatcaatgca ggtaccccca ttctggcagg gctgggacag gcactcgttg atctcctctg
     7321 agcagttaga cccatggtag ccagccacac acttgcagga aaagccgccg agatagtcag
     7381 tgcaggtagc tccattctgg caggggttag gtgagcactc gtccacctcg tcctcacagt
     7441 agctgcccgt gtagcctgcc tggcagtggc agtaatgttt atctccctca tccacacaga
     7501 gccctccatg ctggcacagg agagtgacgt caatgcctcg cttctgtgca gccacctcac
     7561 aggacacact gagcacgtcg cagttgacgc cagtccagcc gctgcggcac tcacagtggt
     7621 actgcgtgtt ggtctgccag cacctgccac cattcttgca gggagccgag tcgcaccagc
     7681 gcacaaggtt ctggcagttg agaccagtgt agccctgtgg gcaggtacac ttataagtac
     7741 catagctgtc ttggcaggta ccaccgtgca gacagggccg tgaatcacac tcattgacat
     7801 catactgaca gtagctgccc gtgaagccag gtggacacag acaggtgaag gagttgatac
     7861 catccacaca ggtaccacca ttgaagcagg agctctcagt acagtcaggt gtgttgttct
     7921 cgcagtggat gccattgaag cccacggggc aggtacatgt gtagctgtcc acacagtcag
     7981 tgcaattggc accattttgg cagggattgc tggcacattc attgatgtcc tcctcacaga
     8041 aggcaccctg gaagccgggc aggcagtcgc agaaggctgt gttgatgcca tcggtgcagg
     8101 aacccccatt gtgacacggg ttggggcggc agtcatcgat gtcactctca cagttgcgac
     8161 ctgtatagcc ggcctggcag aggcagcggt agctgccatt ggtgttctgg caggaggccc
     8221 catggcgaca tgggcttttc acacactcat tgatgtcaac ctcgcaggtt tgaccttgcc
     8281 agcctgtggg acagacacag gaaaaactct catagtcttc agactccttg cataccccgc
     8341 tgtttttgca ggggctggta gcacatgggg ccaacaccac ctcacacgtg gctcctgtat
     8401 atggcagagg acagttgcac ttgtatccag cgacatcatc aatgcaggtc ccctggttca
     8461 ggcaggggtt ggaggcacat tcgttgatgt tggtctggca attagggcca ctgaagcctt
     8521 ctcggcaggt gcatacgtag ccactggtca tgtccttgca ggtgccaccg ttgacacaag
     8581 ggttggactc acactcgttg ttgttgatgt cacagtttgt tccactccac ccaggggcac
     8641 agtcacactt gtacccattg aggccatccc ggcaagctcc gtggatgcag gggttactgt
     8701 tgcactcgtt gacctcggac aggcacgtgg ggtcatggta gccctcgggg cagcggcaag
     8761 tgaagcccgc gatgccatcc tcacaagtgc ccccgttgtg gcaggggctg cccgcacatt
     8821 cgtcaatgtt gacgttacac atgcttcctg tgtagcctgg ttcacaggca cattcgtagc
     8881 catcaatctt gtccagacag gtgccagagt cacaggggtt gctggcgcag tcatccaggt
     8941 tgatctcaca gttgggccct gtggttccct tgaggcataa gcagaggtag gagttgtcac
     9001 ggtcctggca ggtgccccca tggcggcacg gttggctgtg gcactcattg atgttggtct
     9061 cacagtgatg gcctgtgtag cctggctggc acaggcaggt aaaggtggcc acaccatcct
     9121 tacaggaacc atagtggcag gggtcagggt cacactcgtc aatgtccact tcgcagtggg
     9181 tccctgtgta accttctgta cacacgcagg tataggtgtt gggcccatcc aggcacttgg
     9241 caccgttctt gcatggtgtg ctggcacact catccacatc atactggcac aggtgcccgt
     9301 tgaagccttt ggggcactga cattggaact cattgatctt gtccatgcag tggccattgt
     9361 gcagacaggg gctgctggcg cactcatccg tgttgatttc acagtataca ccttcataac
     9421 ctggcataca tatgcattgg aactccccaa tctggtccag gcaagtggca tcattctgac
     9481 atgggttgga gatgcactca ttaacatcaa tctcacagcg gggtcccgtg tagccctgta
     9541 gacactggca ctcaaaagaa cccagtgtgt tgaggcattt gcctgcgtgc tcacaagggt
     9601 tggcacccag agcacactca tccacgtcct ggctgcaggc tggccctgtg taccccgagg
     9661 ggcaggtgca gatggctttg ccgttgacag ggttggtgtc acagttggag ccctcgttgc
     9721 aggggttgct gatgcacgca tcgttgaggt ggcacagcag acctgtgcgc ccatgcggac
     9781 attcgcagta gaaggaagcc acacggtcgt ggcaagtggc accctggaaa caggcggcac
     9841 tggcacagtc atcaatgttc tcactgcagt cctcgccagt ccacccattg acacacacac
     9901 agttgtagcc gccgtgtgtg ttgtggcagg ttccgccatt ctggcaggca ttgggcatga
     9961 gctgacattc gtccacatcc tctgtacagt actgacccgt ccactccggt gggcagcggc
    10021 aattgtaggt attcacgccg tccacacagg cacccccatt cttgcagttg tttcctggac
    10081 agtcatccac attttcttca cagttctgtc cagcaaaacc tggcaagcag gcacactcgt
    10141 gggtggtgtc ccctgtagga cggcaggtgc ctccattctg gcagggtgag gggctgcagg
    10201 gcacataggg cagttcacag tggggaccag tatgggtggc acggcaggca cagcgatagg
    10261 agccgatctc attgtggcag gtgcctccat ggcggcacag cccagggttc tggctgcact
    10321 cattaacatc ttgcctgcag gtggggccat ggaagccagg cgggcagcga cagatgtatg
    10381 aagactcaaa gggcaggcac tggccaccat tggcacaggg gttggaggca caggggtcag
    10441 cctgctgaca tgattttcct gaccaccctg gtgggcagcg gcacttgtac tctgtgagag
    10501 tgagcaggtc acaggtgccc ccattgcggc aggggttggc caggcaggcg ttgtccagag
    10561 gtgtcaggca gaggggccca gagaaaccca ggggacagct gcaggcataa tccacagtgc
    10621 caccatggtc cacaacgtgg cacgttccag cattcttaca cggtgtgctg aggcaaggat
    10681 tggagtcctg gcatcgttgg cccacaaagg ctccgctgca gacacaggct tcagtgccgc
    10741 tggccacttc gcacctacct ccattcaggc aggtcccact tggctgggag catctcaagc
    10801 ctcttgcggc gagcgcgggc agcagcgtta ggcagagcaa gggcgtcagg agccgtggca
    10861 tgcctgctcg ccagctgccc gcagcccggg cggcggcctc ttggggtggc cggcggggat
    10921 cggacgttga cgcacaccac cggagcccag cgccgcccac ccggaattcg aatcgatggg
    10981 atcctgcaaa aagaacaagt agcttgtatt ctatagtgtc acctaaatca agcttgctcc
    11041 ga
//
