LOCUS       sequence-133352-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of Mac-GFP under the Syn promoter, in 
            floxed/reversed (Cre-dependent) manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Chow BY, Han X, Dobry AS, Qian X, Chuong AS, Li M, Henninger MA, 
            Belfort GM, Lin Y, Monahan PE, Boyden ES
  TITLE     High-performance genetically targetable optical neural silencing by 
            light-driven proton pumps.
  JOURNAL   Nature. 2010 Jan 7. 463(7277):98-102.
  PUBMED    20054397
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jan 7. 463(7277):98-102."
COMMENT     Sequence Label: pAAV-Syn-FLEX-Mac-GFP
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    95..128
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 ataatgtatg ctatacgaag ttatgggtcg atggtgagat ctggactaga gggtcgatgg
       61 tgatgcttgg atcaaaatag gaagaatccc aatgataact tcgtataaag tatcctatac
      121 gaagttatac taggtaccgc caccatgatc gtggaccagt tcgaggaggt gctgatgaag
      181 accagccagc tgttcccact gccaaccgct acccagagcg cccagccaac ccacgtggcc
      241 cccgtgccaa ccgtgctgcc cgacaccccc atctacgaga ccgtgggcga cagcggcagc
      301 aagaccctgt gggtggtgtt cgtgctgatg ctgatcgcca gcgccgcctt caccgccctg
      361 agctggaaga tccccgtgaa caggaggctg taccacgtga tcaccaccat catcaccctg
      421 accgccgccc tgagctactt cgctatggct accggccacg gagtggccct gaacaagatc
      481 gtgatcagga cccagcacga ccacgtgccc gacacctacg agaccgtgta ccgacaggtg
      541 tactacgcca ggtacatcga ctgggctatc accaccccac tgctgctgct ggacctggga
      601 ctgctggctg gaatgagcgg agcccacatc ttcatggcca tcgtggctga cctgatcatg
      661 gtgctgaccg gcctgttcgc tgctttcggc agcgagggaa ccccacagaa gtggggatgg
      721 tacaccattg
//
