LOCUS       sequence-133357-        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of ArchT-GFP under the EF1a promoter, in 
            floxed/reversed (Cre-dependent) manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Han X, Chow BY, Zhou H, Klapoetke NC, Chuong A, Rajimehr R, Yang A, 
            Baratta MV, Winkle J, Desimone R, Boyden ES
  TITLE     A high-light sensitivity optical neural silencer: development and 
            application to optogenetic control of non-human primate cortex.
  JOURNAL   Front Syst Neurosci. 2011;5:18. Epub 2011 Apr 13.
  PUBMED    21811444
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Syst 
            Neurosci."; date: "2011"; volume: "5"; pages: "18. Epub 2011 Apr 13"
COMMENT     Sequence Label: pAAV-EF1a-FLEX-ArchT-GFP
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..90
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    161..194
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      205..214
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1012..1033)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttcgcg aacctcttcg agggacataa
       61 cttcgtataa tgtatgctat acgaagttat gggtcgatgg tgagatctgg actagagggt
      121 cgatggtgat gcttggatca aaataggaag aatcccaatg ataacttcgt ataaagtatc
      181 ctatacgaag ttatactagg taccgccacc atggacccca tcgctctgca ggcaggatac
      241 gacctgctgg gcgacggaag gccagagacc ctgtggctgg gaatcggaac cctgctgatg
      301 ctgatcggca ccttctactt catcgtgaag ggctggggcg tgaccgacaa ggaggccagg
      361 gagtactaca gcatcacaat cctggtgccc ggcatcgcca gcgccgccta cctgagcatg
      421 ttcttcggca tcggcctgac cgaggtgacc gtggccggcg aggtgctgga catctactac
      481 gccagatacg ccgactggct gttcaccacc cctttgcttc tgctcgacct ggccctgctg
      541 gctaaggtgg acagggtgag catcggaacc ctggtgggag tggacgccct gatgatcgtg
      601 accggcctga tcggcgccct gagccacacc ccactggcta ggtacagctg gtggctgttc
      661 agcaccatct gcatgatcgt ggtgctgtac ttcctggcta ccagcctgag ggctgctgct
      721 aaggagaggg gaccagaggt ggctagcacc ttcaacaccc tgaccgccct ggtgctggtg
      781 ctgtggaccg cctaccccat cctgtggatc atcggaaccg agggagctgg agtggtggga
      841 ctgggaatcg agaccctgct gttcatggtg ctggacgtga ccgccaaagt gggcttcggc
      901 ttcatcctgc tgaggagcag ggccatcctg ggcgacaccg aggcccccga gccccgaccg
      961 gtagtagcag tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     1021 ctggacggcg acgtaaacgg ccacaagtt
//
