LOCUS       sequence-133359-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of ChR2-mCherry under the GFAP promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Perea G, Yang A, Boyden ES, Sur M
  TITLE     Optogenetic astrocyte activation modulates response selectivity of 
            visual cortex neurons in vivo.
  JOURNAL   Nat Commun. 2014;5:3262. doi: 10.1038/ncomms4262.
  PUBMED    24500276
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014"; volume: "5:3262. doi"; pages: " 
            10.1038/ncomms4262"
COMMENT     Sequence Label: pAAV-GFAP104-ChR2-mCherry
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      52..61
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             58..984
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             58..984
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
ORIGIN
        1 ccagagcagg ttggagagga gacgcatcac ctccgctgct cgcggggatc cgccaccatg
       61 gactatggcg gcgctttgtc tgccgtcgga cgcgaacttt tgttcgttac taatcctgtg
      121 gtggtgaacg ggtccgtcct ggtccctgag gatcaatgtt actgtgccgg atggattgaa
      181 tctcgcggca cgaacggcgc tcagaccgcg tcaaatgtcc tgcagtggct tgcagcagga
      241 ttcagcattt tgctgctgat gttctatgcc taccaaacct ggaaatctac atgcggctgg
      301 gaggagatct atgtgtgcgc cattgaaatg gttaaggtga ttctcgagtt cttttttgag
      361 tttaagaatc cctctatgct ctaccttgcc acaggacacc gggtgcagtg gctgcgctat
      421 gcagagtggc tgctcacttg tcctgtcatc cttatccacc tgagcaacct caccggcctg
      481 agcaacgact acagcaggag aaccatggga ctccttgtct cagacatcgg gactatcgtg
      541 tggggggcta ccagcgccat ggcaaccggc tatgttaaag tcatcttctt ttgtcttgga
      601 ttgtgctatg gcgcgaacac attttttcac gccgccaaag catatatcga gggttatcat
      661 actgtgccaa agggtcggtg ccgccaggtc gtgaccggca tggcatggct gtttttcgtg
      721 agctggggta tgttcccaat tctcttcatt ttggggcccg aaggttttgg cgtcctgagc
      781 gtctatggct ccaccgtagg tcacacgatt attgatctga tgagtaaaaa ttgttggggg
      841 ttgttgggac actacctgcg cgtcctgatc cacgagcaca tattgattca cggagatatc
      901 cgcaaaacca ccaaactgaa catcggcgga acggagatcg aggtcgagac tctcgtcgaa
      961 gacgaagccg aggccggagc cgtgccagcg gcaccggtag tgagcaaggg cgaag
//
