LOCUS       sequence-133362-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This E. coli DH10B strain harbors a shuttle vector plasmid that 
            expresses the Mixed Feedback Loop version of the UBER system with a 
            T7RNAP translation rate of 1984 and a TetR translation rate of 
            3877..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Kushwaha M, Salis HM
  TITLE     A portable expression resource for engineering cross-species genetic
            circuits and pathways.
  JOURNAL   Nat Commun. 2015 Jul 17;6:7832. doi: 10.1038/ncomms8832.
  PUBMED    26184393
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-17"; volume: "6:7832. doi"; pages: " 
            10.1038/ncomms8832"
COMMENT     Sequence Label: MK1278
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     187..206
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        187..205
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             294..314
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 acacatgatc atatggcatg catgtgctct gtatgtatat aaaactcttg ttttcttctt
       61 ttctctaaat attctttcct tatacattag gtcctttgta gcataaatta ctatacttct
      121 atagacacgc aaacacaaat acacacacta aattaataac tagttctatc attgataggt
      181 ataaattaat acgactcact atagggagac ctatcagtga tagaggatcc aaccgagccc
      241 aatataggac ctatggagcc aaaaaaatgt ctaccaccat ggagatctat cctcctaaaa
      301 aaaaacgtaa agtttcacgt ttagataaat caaaagttat taactcagca ttagaattat
      361 taaatgaagt tggtatcgaa ggtttaacta cacgtaaatt agcacagaaa ttaggtgttg
      421 aacaacctac attatattgg catgttaaaa acaaacgtgc attattagat gcattagcaa
      481 tcgaaatgtt agatagacat catacacatt tttgtccatt agaaggagaa tcatggcaag
      541 acttcttacg taacaatgcc aaatcatttc gttgtgcatt attatcacat agagatggtg
      601 ccaaagttca cttaggtaca cgtcctacag aaaaacaata tgaaacatta gaaaatcagt
      661 tagcattctt atgtcaacaa ggtttttcat tagaaaacga atttaaatta taattatttt
      721 tatagcacgt gatgaaaagg acc
//
