LOCUS       sequence-133363-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of ChR2(H134R)-GFP under the Syn promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K
  TITLE     Millisecond-timescale, genetically targeted optical control of 
            neural activity.
  JOURNAL   Nat Neurosci. 2005 Sep . 8(9):1263-8.
  PUBMED    16116447
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2005 Sep . 8(9):1263-8."
COMMENT     Sequence Label: pAAV-Syn-ChR2(H134R)-GFP
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(38..964)
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     regulatory      961..970
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctcctcgcc cttgctcact gctactaccg gtgccgccac ggctccggcc tcggcttcgt
       61 cttcgacgag agtctcgacc tcgatctccg ttccgccgat gttcagtttg gtggttttgc
      121 ggatatctcc gtgaatcaat atgtgctcgt ggatcaggac gcgcaggtag tgtcccaaca
      181 acccccaaca atttttactc atcagatcaa taatcgtgtg acctacggtg gagccataga
      241 cgctcaggac gccaaaacct tcgggcccca aaatgaagag aattgggaac ataccccagc
      301 tcacgaaaaa cagccatgcc atgccggtca cgacctggcg gcaccgaccc tttggcacag
      361 tatgataacc ctcgatatat gctttggcgg cgtgaaaaaa tgtgttcgcg ccatagcaca
      421 atccaagaca aaagaagatg actttaacat agccggttgc catggcgctg gtagcccccc
      481 acacgatagt cccgatgtct gagacaagga gtcccatggt tctcctgctg tagtcgttgc
      541 tcaggccggt gaggttgctc agtctgataa ggatgacagg acaagtgagc agccactctg
      601 catagcgcag ccactgcacc cggtgtcctg tggcaaggta gagcatagag ggattcttaa
      661 actcaaaaaa gaactcgaga atcaccttaa ccatttcaat ggcgcacaca tagatctcct
      721 cccagccgca tgtagatttc caggtttggt aggcatagaa catcagcagc aaaatgctga
      781 atcctgctgc aagccactgc aggacatttg acgcggtctg agcgccgttc gtgccgcgag
      841 attcaatcca tccggcacag taacattgat cctcagggac caggacggac ccgttcacca
      901 ccacaggatt agtaacgaac aaaagttcgc gtccgacggc agacaaagcg ccgccatagt
      961 ccatggtggc ggatcctctc gactgcgctc tcaggcacga
//
