LOCUS       sequence-133367-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This E. coli DH10B strain harbors a shuttle vector plasmid that 
            expresses the Mixed Feedback Loop version of the UBER system with a 
            T7RNAP translation rate of 300 and a TetR translation rate of 
            35149..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Kushwaha M, Salis HM
  TITLE     A portable expression resource for engineering cross-species genetic
            circuits and pathways.
  JOURNAL   Nat Commun. 2015 Jul 17;6:7832. doi: 10.1038/ncomms8832.
  PUBMED    26184393
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-17"; volume: "6:7832. doi"; pages: " 
            10.1038/ncomms8832"
COMMENT     Sequence Label: MK1274
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(713..733)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagctattaa ttaaacaaaa aacccctacc cccccgatat gtatttattg cgaaaaaacc
       61 ccgccgaagc ggggtttttt gcgaccggtt attatgaacc tgattcacat tttaactgtt
      121 tctccaaacc gcaaatgatt aattctaaac caaataaaaa tgcaggttct gcaccttgat
      181 gatcaaataa ttcaattgct tgacgtaata aaggaggcat tgaatctgtt gtaggtgttt
      241 ctctttcctc cttggcaact tgatgttctt gatcttctaa aacacaacct aaggtaaaat
      301 gaccaactgc tgataatgca tataatgcgt tttctaatga aaaaccttgt tgacataaga
      361 atgctaactg attttctaat gtttcatatt gtttttctgt aggacgtgta cctaagtgaa
      421 ctttggcacc atctctatgt gataataatg cacaacgaaa tgatttggca ttgttacgta
      481 agaagtcttg ccatgattct ccttctaatg gacaaaaatg tgtatgatgt ctatctaaca
      541 tttcgattgc taatgcatct aataatgcac gtttgttttt aacatgccaa tataatgtag
      601 gttgttcaac acctaatttc tgtgctaatt tacgtgtagt taaaccttcg ataccaactt
      661 catttaataa ttctaatgct gagttaataa cttttgattt atctaaacgt gaaactttac
      721 gttttttttt aggaggatag atctccatgg tggtagacat ttttgaccgc ccttagaatt
      781 cggcgcgtgc tggtgccccg ggatagccct atagtgagtc gta
//
