LOCUS       sequence-133368-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: pCVL Traffic Light Reporter 2.1 (VF2468 ZFN ta...
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(75..782)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNSAIIKEFLRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(353..372)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     622..640
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(783..836)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tgtgggcgat gtgcgctctg cccactgacg ggcaccggag cctctgcagc tagaatcagt
       61 ccggactaat gcattcactt gtacagctcg tccatgccgc cggtggagtg gcggccctcg
      121 gcgcgttcgt actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccaacttg
      181 atgttgacgt tgtaggcgcc gggcagctgc acgggcttct tggccttgta ggtggtcttg
      241 acctcagcgt cgtagtggcc gccgtccttc agcttcagcc tctgcttgat ctcgcccttc
      301 agggcgccgt cctcggggta catccgctcg gaggaggcct cccagcccat ggtcttcttc
      361 tgcattacgg ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt gtagatgaac
      421 tcgccgtcct gcagagagga gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc
      481 atcacgcgct cccacttgaa gccctcgggg aaggacagct tcaagtagtc ggggatgtcg
      541 gcggggtgct tcacgtaggc cttggagccg tacatgaact gaggggacag gatgtcccag
      601 gcgaagggca gggggccacc cttggtcacc ttcagcttgg cggtctgggt gccctcgtag
      661 gggcggccct cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg
      721 tgcaccttga agcgcaggaa ctccttgatg atggcggagt tatcctcctc gcccttgctc
      781 acagggcccg gattctcctc cacgtcaccg catgttagaa gacttcctct gccctcaccc
      841 acgttgatcc tagcgctggc gcaagcggcg gggtgcctgt ccatgccgct ctcctgggcg
      901 cagctcatgg gcagggtgcc atcgtcct
//
