LOCUS       sequence-133370-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral backbone for cloning and expression of new shRNA 
            sequences. Uses neomycin for selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  TITLE     Stewart Lab Plasmids
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLKO.1 neo
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             74..307
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     389..405
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     390..406
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(539..558)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ttcctctcac tctctgatat tcatttcttt gcaagttata aatactgaat aataagatga
       61 catgaactac tactgctaga gattttccac actgactaaa agggtctgag ggatctctag
      121 ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat
      181 tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct
      241 aaccagagag acccagtaca agcaaaaagc agatcttgtc ttcgttggga gtgaattagc
      301 ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc
      361 attggtctta aaggtaccgg gccccccctc gaggtcgacg gtatcgatgc gaaccccaga
      421 gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag
      481 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa
      541 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt
      601 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat
      661 gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg
      721 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca
      781 tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg
      841 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag
      901 caaggtgaga tgacaggaga tcctgc
//
