LOCUS       sequence-133373-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for the analysis of miR-193a-5p 
            activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Shen J, Xia W, Khotskaya YB, Huo L, Nakanishi K, Lim SO, Du Y, Wang 
            Y, Chang WC, Chen CH, Hsu JL, Wu Y, Lam YC, James BP, Liu X, Liu CG,
            Patel DJ, Hung MC
  TITLE     EGFR modulates microRNA maturation in response to hypoxia through 
            phosphorylation of AGO2.
  JOURNAL   Nature. 2013 May 16;497(7449):383-7. doi: 10.1038/nature12080. Epub 
            2013 May 1.
  PUBMED    23636329
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12080"; journalName: "Nature"; date: "2013-05-16- 
            16"; volume: "497"; issue: "7449"; pages: "383-7"
COMMENT     Sequence Label: pMIR-193a-5p-Reporter
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(204..221)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(204..220)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(213..235)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(429..448)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     548..570
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa
       61 gaagggcgga aagtccaaat tgctcgagtg atgaaagctg cgcactagtt catctcgccc
      121 gcaaagaccc aaagcttaat aaaggatctt ttattttcat tggatctgtg tgttggtttt
      181 ttgtatgcgg ccgctagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac
      241 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      301 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      361 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
      421 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
      481 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
      541 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
      601 cgaaagattg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      661 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
      721 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
      781 cgcccctaac tccgcc
//
