LOCUS       sequence-133374-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral coexpression of Cre and puromycin from the human EF1a 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Somers A, Jean JC, Sommer CA, Omari A, Ford CC, Mills JA, Ying L, 
            Sommer AG, Jean JM, Smith BW, Lafyatis R, Demierre MF, Weiss DJ, 
            French DL, Gadue P, Murphy GJ, Mostoslavsky G, Kotton DN
  TITLE     Generation of transgene-free lung disease-specific human induced 
            pluripotent stem cells using a single excisable lentiviral stem cell
            cassette.
  JOURNAL   Stem Cells. 2010 Oct . 28(10):1728-40.
  PUBMED    20715179
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2010 Oct . 28(10):1728-40."
COMMENT     Sequence Label: Cre-IRES-PuroR
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcggccgcc atgggcacac gactccccaa gaagaggaag gtgtccaatt tactgaccgt
       61 acaccaaaat ttgcctgcat taccggtcga tgcaacgagt gatgaggttc gcaagaacct
      121 gatggacatg ttcagggatc gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc
      181 cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac cggaaatggt ttcccgcaga
      241 acctgaagat gttcgagatt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac
      301 tatccagcaa catttgggcc agctaaacat gcttcatcgt cggtccgggc tgccacgacc
      361 aagtgacagc aatgctgttt cactggttat gcggcggatc cgaaaagaaa acgttgatgc
      421 cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc
      481 actcatggaa aatagcgatc gctgccagga tatacgtaat ctggcatttc tggggattgc
      541 ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag atatctcacg
      601 tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc
      661 aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt
      721 ctctggtgta gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt
      781 tgccgcgcca tctgccacca gccagctatc aactcgcgc
//
