LOCUS       sequence-133377-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCI HA NEDD4
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        23..41
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             62..88
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cagctcttaa ggctagagta cttaatacga ctcactatag gctagcctcg agaccatgga
       61 ttatccgtat gatgttcctg attatgcaga attcacgcgt ggtaccgcaa cttgcgcggt
      121 ggaggtgttc gggctcctgg aggacgagga aaattcacga attgtgagag taagagttat
      181 agccggaata ggccttgcca agaaggatat attgggagct agtgatcctt acgtgagagt
      241 gacgttatat gacccaatga atggagttct tacaagtgtg caaacaaaaa ccattaaaaa
      301 gagtttgaat ccaaagtgga atgaagaaat attattcaga gttcatcctc agcagcaccg
      361 gcttcttttt gaagtgtttg acgaaaaccg attgacaaga gatgatttcc taggtcaagt
      421 ggatgttcca ctttatccat taccgacaga aaatccaaga ttggagagac catatacatt
      481 taaggatttt gttcttcatc caagaagtca caaatcaaga gttaaaggtt atctgagact
      541 aaaaatgact tatttaccta aaaccagtgg ctcagaagat gataatgcag aacaggctga
      601 ggaattagag cctggctggg ttgttttgga ccaaccagat gctgcttgcc atttgcagca
      661 acaacaagaa ccttctcctc tacctccagg gtgggaagag aggcaggata tccttggaag
      721 gacctattat gtaaaccatg aatctagaag aacacagtgg aaaagaccaa cccctcagga
      781 caacctaaca gatgctgaga atggcaacat tcaactgcaa gcacaacgtg catttaccac
      841 caggcggcag atatccgagg aaacaga
//
