LOCUS       sequence-133380-         524 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of Chronos-GFP under the Syn promoter, in 
            floxed/reversed (Cre-dependent) manner. Using bGHpA signal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK,
            Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, 
            Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, 
            Constantine-Paton M, Wong GK, Boyden ES
  TITLE     Independent optical excitation of distinct neural populations.
  JOURNAL   Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 
            2014 Feb 9.
  PUBMED    24509633
REFERENCE   2  (bases 1 to 524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2836"; journalName: "Nat Methods"; date: "2014-03"; 
            volume: "11"; issue: "3"; pages: "338-46"
COMMENT     Sequence Label: pAAV-Syn-FLEX-rc[Chronos-GFP]
FEATURES             Location/Qualifiers
     source          1..524
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    53..86
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      108..117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggactagagg gtcgatggtg atgcttggat caaaatagga agaatcccaa tgataacttc
       61 gtataaagta tcctatacga agttatacta ggtacctagg ccgcgccgcc accatggaaa
      121 cagccgccac aatgacccac gcctttatct cagccgtgcc tagcgccgaa gccacaatta
      181 gaggcctgct gagcgccgca gcagtggtga caccagcagc agacgctcac ggagaaacct
      241 ctaacgccac aacagccgga gccgatcacg gttgcttccc ccacatcaac cacggaaccg
      301 agctgcagca caagatcgca gtgggactcc agtggttcac cgtgatcgtg gctatcgtgc
      361 agctcatctt ctacggttgg cacagcttca aggccacaac cggctgggag gaggtctacg
      421 tctgcgtgat cgagctcgtc aagtgcttca tcgagctgtt ccacgaggtc gacagcccag
      481 ccacagtgta ccagaccaac ggaggagccg tgatttggct gcgg
//
