LOCUS       sequence-133396-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses thiol redox-sensitive ratiometric sensor roGFP in the 
            mitochondrial intermembrane space of mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Waypa GB, Marks JD, Guzy R, Mungai PT, Schriewer J, Dokic D, 
            Schumacker PT
  TITLE     Hypoxia triggers subcellular compartmental redox signaling in 
            vascular smooth muscle cells.
  JOURNAL   Circ Res. 2010 Feb 19;106(3):526-35. doi: 
            10.1161/CIRCRESAHA.109.206334. Epub 2009 Dec 17.
  PUBMED    20019331
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1161/CIRCRESAHA.109.206334"; journalName: "Circ Res"; date: 
            "2010-02-19- 19"; volume: "106"; issue: "3"; pages: "526-35"
COMMENT     Sequence Label: GPD-roGFP
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      470..479
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctc gtttagtgac cgtcagatgg taccgtttaa actcgagcgg ccgccagtgt
       61 gatggatatc tgcagaattc cgctcgccga cccctggcgt gtgacgaccg atctcaggct
      121 gagcaatggc gtttcaaaag gtagtgaaag ggactattct tatgggtgga ggagctctgg
      181 ccactgtttt gggactctct cagtttgctc actacagaag gaagcaagta agcctggcgt
      241 atgtggaagc agcaacatgc ttctcagagc ccgtgaacag ggagcctcct tccagagaag
      301 ctcagctcat gactctgcag aacacatcgg aatttgacat cctcgtcatt ggaggaggag
      361 ccacaggatg tggctgtgcg ctagacgccg tcaccagagg actgaaaaca gcccttgtag
      421 agagaaatga cttcgcatca gggactagca gcagaagcac taaattgatc catggtggcg
      481 tacgatacct ccagaaggct atcacgaact tggatgttga acagtatagg atggtgaaag
      541 aggcccttca tgaacgtgcc aacttactag aaatcgctcc ccatctatca gctccgctgc
      601 ctataatgct gccactttat aagtggtggc agttacctta ttactgggtg ggaatcaaga
      661 tgtatgatct ggttgcagga agtcattgcc tgaagagcag tt
//
