LOCUS       sequence-133407-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the amyloid-beta protein (42 aa), containing an exogenous 
            methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. 
            coli cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Walsh DM, Thulin E, Minogue AM, Gustavsson N, Pang E, Teplow DB, 
            Linse S
  TITLE     A facile method for expression and purification of the Alzheimer's 
            disease-associated amyloid beta-peptide.
  JOURNAL   FEBS J. 2009 Mar;276(5):1266-81. doi: 
            10.1111/j.1742-4658.2008.06862.x.
  PUBMED    19175671
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1742-4658.2008.06862"; journalName: "FEBS J"; date: 
            "2009-03"; volume: "276"; issue: "5"; pages: "1266-81"
COMMENT     Sequence Label: pET-Sac-Abeta(M1-42)
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             159..181
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(212..231)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(213..231)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(315..334)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cagccggatc gagctcctat taagcgatca caacgccacc aaccatcaga ccgatgatag
       61 cacccttgtt agaacccacg tcttcagcga agaacaccag cttctggtgg tgaacttcgt
      121 aaccagagtc gtgacggaat tcagcgtcca tatgtatatc tccttcttaa agttaaacaa
      181 aattatttct agagggaaac cgttgtggtc tccctatagt gagtcgtatt aatttcgcgg
      241 gatcgagatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
      301 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
      361 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
      421 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
      481 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaccgatgcc
      541 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
      601 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg
      661 ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc
      721 ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg
      781 tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt
      841 gctggcgttc gcgacgcgag gctggatggc cttcc
//
