LOCUS       sequence-133408-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the amyloid-beta protein (40 aa), containing an exogenous 
            methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. 
            coli cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Walsh DM, Thulin E, Minogue AM, Gustavsson N, Pang E, Teplow DB, 
            Linse S
  TITLE     A facile method for expression and purification of the Alzheimer's 
            disease-associated amyloid beta-peptide.
  JOURNAL   FEBS J. 2009 Mar;276(5):1266-81. doi: 
            10.1111/j.1742-4658.2008.06862.x.
  PUBMED    19175671
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1742-4658.2008.06862"; journalName: "FEBS J"; date: 
            "2009-03"; volume: "276"; issue: "5"; pages: "1266-81"
COMMENT     Sequence Label: pET-Sac-Abeta(M1-40)
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             154..176
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(207..226)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(208..226)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(310..329)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcagccggat cgagctccta ttacacaacg ccaccaacca tcagaccgat gatagcaccc
       61 ttgttagaac ccacgtcttc agcgaagaac accagcttct ggtggtgaac ttcgtaacca
      121 gagtcgtgac ggaattcagc gtccatatgt atatctcctt cttaaagtta aacaaaatta
      181 tttctagagg gaaaccgttg tggtctccct atagtgagtc gtattaattt cgcgggatcg
      241 agatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
      301 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
      361 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
      421 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
      481 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaccg atgcccttga
      541 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
      601 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
      661 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
      721 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
      781 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
      841 cgttcgcgac gcgaggctgg atggccttcc
//
