LOCUS       sequence-133418-        1122 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A crRNA expression plasmid for targeting a specific sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA
  TITLE     RNA-guided editing of bacterial genomes using CRISPR-Cas systems.
  JOURNAL   Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508.
  PUBMED    23360965
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508."
COMMENT     Sequence Label: pCRISPR
FEATURES             Location/Qualifiers
     source          1..1122
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..227
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(248..266,267..272,273..289,290..295,296..321)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    248..266
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    273..291
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     repeat_region   354..389
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   420..455
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     misc_feature    complement(456..587)
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   628..663
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   694..729
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     misc_feature    complement(730..861)
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     terminator      891..977
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc
       61 agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt
      121 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccgacg tctaagaaac
      181 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
      241 cctcgagtcc ctatcagtga tagagattga catccctatc agtgatagag atactgagca
      301 catcagcagg acgcactgac cgaattcaac tcaacaagtc tcagtgtgct gaagttttgg
      361 gaccattcaa aacagcatag ctctaaaacg agacctttga gcttccgaga ctggtctcag
      421 ttttgggacc attcaaaaca gcatagctct aaaacctcgt agactatttt tgtctaaaaa
      481 atttcgtaat cgcactattt gtctcagcta gacttcagtc ttgaaaagcc cctgtattac
      541 tgcatttatt aagagtatta taccatattt ttagttatta agaaatagga tccatgaatt
      601 caactcaaca agtctcagtg tgctgaagtt ttgggaccat tcaaaacagc atagctctaa
      661 aacgagacct ttgagcttcc gagactggtc tcagttttgg gaccattcaa aacagcatag
      721 ctctaaaacc tcgtagacta tttttgtcta aaaaatttcg taatcgcact atttgtctca
      781 gctagacttc agtcttgaaa agcccctgta ttactgcatt tattaagagt attataccat
      841 atttttagtt attaagaaat aggatcccat ggtacgcgtg ctagaggcat caaataaaac
      901 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      961 tcctgagtag gacaaatccg ccgccctaga cctagggcgt tcggctgcgg cgagcggtat
     1021 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1081 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aa
//
