LOCUS       sequence-133421-        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Kanda T, Sullivan KF, Wahl GM
  TITLE     Histone-GFP fusion protein enables sensitive analysis of chromosome 
            dynamics in living mammalian cells.
  JOURNAL   Curr Biol. 1998 Mar 26. 8(7):377-85.
  PUBMED    9545195
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            1998 Mar 26. 8(7):377-85."
COMMENT     Sequence Label: H2B-GFP
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             98..475
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      488..497
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(494..496,497..499,500..1012)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(539..560)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(800..819)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
       61 tcaagcttcg aattctgcag tcgacggtac cgccaccatg ccagagccag cgaagtctgc
      121 tcccgccccg aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa
      181 gaagcgcaag cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca
      241 ggtccaccct gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa
      301 cgacattttc gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc
      361 gaccatcacc tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc
      421 caagcacgcc gtgtccgagg gtactaaggc catcaccaag tacaccagcg ctaaggatcc
      481 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      541 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      601 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      661 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
      721 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      781 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      841 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      901 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
      961 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     1021 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1081 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgaga
//
