LOCUS       sequence-133422-         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of aa 518-860 of human Ago2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Shen J, Xia W, Khotskaya YB, Huo L, Nakanishi K, Lim SO, Du Y, Wang 
            Y, Chang WC, Chen CH, Hsu JL, Wu Y, Lam YC, James BP, Liu X, Liu CG,
            Patel DJ, Hung MC
  TITLE     EGFR modulates microRNA maturation in response to hypoxia through 
            phosphorylation of AGO2.
  JOURNAL   Nature. 2013 May 16;497(7449):383-7. doi: 10.1038/nature12080. Epub 
            2013 May 1.
  PUBMED    23636329
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12080"; journalName: "Nature"; date: "2013-05-16- 
            16"; volume: "497"; issue: "7449"; pages: "383-7"
COMMENT     Sequence Label: pCMV5-Flag-Ago2-FDM5
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..77
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      462..471
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgtcagaa ttaattcaga tctggtacca cgcgtatcga taagcttacc atggattaca
       61 aggatgacga cgataaggga tccatggtgg tggtcatcct gcccggcaag acgcccgtgt
      121 acgccgaggt caagcgcgtg ggagacacgg tgctggggat ggccacgcag tgcgtgcaga
      181 tgaagaacgt gcagaggacc acgccacaga ccctgtccaa cctctgcctg aagatcaacg
      241 tcaagctggg aggcgtgaac aacatcctgc tgccccaggg caggccgccg gtgttccagc
      301 agcccgtcat ctttctggga gcagacgtca ctcacccccc cgccggggat gggaagaagc
      361 cctccattgc cgccgtggtg ggcagcatgg acgcccaccc caatcgctac tgcgccaccg
      421 tgcgcgtgca gcagcaccgg caggagatca tacaagacct ggccgccatg gtccgcgagc
      481 tcctcatcca gttctacaag tccacgcgct tcaagcccac ccgcatcatc ttctaccgcg
      541 acggtgtctc tgaaggccag ttccagcagg ttctccacca cgagttgctg gccatccgtg
      601 aggcctgtat caagctagaa aaagactacc agcccgggat caccttcatc gtggtgcaga
      661 agaggcacca cacccggctc ttctgcactg acaagaacga gcgggttggg aaaagtggaa
      721 acattccagc aggcacgact gtggacacga aaatcaccca ccccaccgag ttcgacttct
      781 acctgtgtag tcacgctggc atccagg
//
