LOCUS       sequence-133423-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a 
            Cre-dependent virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Stachniak TJ, Ghosh A, Sternson SM
  TITLE     Chemogenetic synaptic silencing of neural circuits localizes a 
            hypothalamus-->midbrain pathway for feeding behavior.
  JOURNAL   Neuron. 2014 May 21;82(4):797-808. doi: 
            10.1016/j.neuron.2014.04.008. Epub 2014 Apr 24.
  PUBMED    24768300
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.04.008"; journalName: "Neuron"; date: 
            "2014-05-21- 21"; volume: "82"; issue: "4"; pages: "797-808"
COMMENT     Sequence Label: AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..98
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    169..202
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      209..218
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattcgaa cctcttcgag
       61 ggacataact tcgtataatg tatgctatac gaagttatgg gtcgatggtg agatctggac
      121 tagagggtcg atggtgatgc ttggatcaaa ataggaagaa tcccaatgat aacttcgtat
      181 aaagtatcct atacgaagtt atactagtgc caccatggac tatggcggcg ctttgtctgc
      241 cgtcggacgc gaacttttgt tcgttactaa tcctgtggtg gtgaacgggt ccgtcctggt
      301 ccctgaggat caatgttact gtgccggatg gattgaatct cgcggcacga acggcgctca
      361 gaccgcgtca aatgtcctgc agtggcttgc agcaggattc agcattttgc tgctgatgtt
      421 ctatgcctac caaacctgga aatctacatg cggctgggag gagatctatg tgtgcgccat
      481 tgaaatggtt aaggtgattc tcgagttctt ttttgagttt aagaatccct ctatgctcta
      541 ccttgccaca ggacaccggg tgcagtggct gcgctatgca gagtggctgc tcacttgtcc
      601 tgtcatcctt atccgcctga gcaacctcac cggcctgagc aacgactaca gcaggagaac
      661 catgggactc cttgtctcag acatcgggac tatcgtgtgg ggggctacca gcgccatggc
      721 aaccggctat gttaaagtca tcttcttttg tcttggattg tgctatggcg cgaacacatt
      781 ttttcacgcc gccaaagcat atatcgaggg ttatcatact gtgccaaagg gtcggtgccg
      841 ccaggtcgtg accggcatgg catggctgtt tttcgtgagc tggggta
//
