LOCUS       sequence-133426-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Zebrafish expression vector for ubiquitous expression of APEX2-GBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    267..291
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      292..301
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctacgactct ggctgagtgc cacaccgccg gcagccgcaa agcggctcaa accattgcct
       61 tttatggtaa taatgagaga atgcagaggg acttcctttg tctggcatat ctgaggcgcg
      121 cgctgtcact agcgcccacc agcggtcaga ctgtagaatg cagagcaaaa caggaagttg
      181 actccagatg gtcacatgct tgctgaaacg cctctccctg gcagcagtgc aattctaaca
      241 gtttcccttt cttttacagt tcagcctcaa gtttgtacaa aaaagcaggc tgccaccatg
      301 ggaaagtctt acccaactgt gagtgctgat taccaggacg ccgttgagaa ggcgaagaag
      361 aagctcagag gcttcatcgc tgagaagaga tgcgctcctc taatgctccg tttggcattc
      421 cactctgctg gaacctttga caagggcacg aagaccggtg gacccttcgg aaccatcaag
      481 caccctgccg aactggctca cagcgctaac aacggtcttg acatcgctgt taggcttttg
      541 gagccactca aggcggagtt ccctattttg agctacgccg atttctacca gttggctggc
      601 gttgttgccg ttgaggtcac gggtggacct aaggttccat tccaccctgg aagagaggac
      661 aagcctgagc caccaccaga gggtcgcttg cccgatccca ctaagggttc tgaccatttg
      721 agagatgtgt ttggcaaagc tatggggctt actgaccaag atatcgttgc tctatctggg
      781 ggtcacacta ttggagctgc acacaaggag cgttctggat ttgagggtcc ctggacctct
      841 aatcctctta ttt
//
