LOCUS       sequence-133433-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT 
            core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA-dCas9-p300 Core (D1399Y)
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..29)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      257..266
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gaagcgtagt ccggaacgtc gtacgggtag tcctggctct gcgtgtgcag ctccaccagc
       61 atgcacatgg tggaccactg ggctcttcgg agtgaagaga actccaggtg cttgtccctt
      121 gccagcgtga gaaacgcatc ccgaccatcc atcagatcgc aggggatgag aggatcagga
      181 tcaacaatgg gaggcaggga gttggcagca gggccagcaa tgaggcggat cacaaagaag
      241 acctctttat gcttctccat ggtggcatat agtttctgtg agaggtcgtt agatacattg
      301 ggcatcccgg gtttcttctt gttgccccta ctcaggctgc tcttattttt gctggttttc
      361 ttattattct tctttttagc atttttgctg tctcccttgg tcacatctgt gctttcattg
      421 ctggtgtttt cctctcgttt tctctcttct tcctcctgtt ccagttcctt aatgctttct
      481 tccagaacat tgggccagaa atcaccctcg aaataaggca attcctttgc acttgttaat
      541 ctatcttcag tagcttgttt aaaaatatcc ttgtagtcat ggacaatacg ctctgataca
      601 gccttgtcaa gcattttttt gtaccattcc tgcagtcgct tgggcttggg tatcttctgg
      661 tcaggaggat ggcaatggaa gatataatca tctccctcac ttggtggaca tgcccaaata
      721 tgccctgttg tgtaacctaa tttcttgaca tattcta
//
