LOCUS       sequence-133436-        1134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses far-red fluorescent protein mCardinal in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: pcDNA3-mCardinal
FEATURES             Location/Qualifiers
     source          1..1134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             104..838
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     promoter        complement(899..917)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(900..917)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(937..954)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    943..1054
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc gagctcggat ccgatggtga gcaagggcga
      121 ggagctgatc aaggagaaca tgcacatgaa gctgtacatg gaaggcaccg tgaacaacca
      181 ccacttcaag tgcaccaccg aaggggaggg caagccctac gagggcaccc agacccagag
      241 gattaaggtg gtggagggag gccccctgcc gttcgcattc gacatcctgg ccacctgctt
      301 tatgtacggg agcaagacct tcatcaacca cacccagggc atccccgatt tctttaagca
      361 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
      421 taccgttacc caggacacca gcctccagga cggctgcttg atctacaacg tcaagctcag
      481 aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
      541 caccaccgag accctgtacc ccgctgacgg cggcctggaa ggcagatgcg acatggccct
      601 gaagctcgtg ggcgggggcc acctgcactg caacctgaag accacataca gatccaagaa
      661 acccgctaag aacctcaaga tgcccggcgt ctactttgtg gaccgcagac tggaaagaat
      721 caaggaggcc gacaatgaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg
      781 cgacctccct agcaaactgg ggcacaaact taatggcatg gacgagctgt acaagtaaga
      841 attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagag ggccctattc
      901 tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc
      961 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     1021 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     1081 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aata
//
